中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
9期
606-609
,共4页
刘太华%刘德芳%陈易华%汪新红%汪晓军%王骏%邓义富%罗陈
劉太華%劉德芳%陳易華%汪新紅%汪曉軍%王駿%鄧義富%囉陳
류태화%류덕방%진역화%왕신홍%왕효군%왕준%산의부%라진
银屑病%默克尔细胞%角蛋白,20
銀屑病%默剋爾細胞%角蛋白,20
은설병%묵극이세포%각단백,20
Psoriasis%Merkel cells%Keratin-20
目的 探讨不同病期银屑病皮损组织中CK20、S100A7、SP的表达及CK20与S100A7、SP表达的关系.方法 选择经过非正规治疗,新旧皮损同时存在的患者19例,获取皮损旁的正常皮肤组织(发病前期)、皮损组织(进展期)和皮损修复后的病灶皮肤组织(缓解期),观察皮损中免疫组化CK20、S100A7、SP的表达情况.结果 免疫组化图像分析各组A值,发病前期组、进展期组和缓解组CK20分别为7683.80±6134.55、18305.04±13171.30、7257.53±4417.75.S100A7分别为8789.05±6240.91、18058.01±16537.18、9295.65±9310.02.SP分另为3242.51±3775.41、9364.98±7596.64、2910.85±3349.46.进展期皮损组织中CK20与S100A7,SP表达相对于发病前期和缓解期明显增加其差异均有统计学意义,P＜0.05.发病前期组与缓解组其差异均无统计学意义,P＞0.05.CK20与S100A7、SP表达呈正相关,相关系数R分别为0.779、0.876、P＜0.05.结论 银屑病发病与Merkel细胞数量的变化有一定的关系.
目的 探討不同病期銀屑病皮損組織中CK20、S100A7、SP的錶達及CK20與S100A7、SP錶達的關繫.方法 選擇經過非正規治療,新舊皮損同時存在的患者19例,穫取皮損徬的正常皮膚組織(髮病前期)、皮損組織(進展期)和皮損脩複後的病竈皮膚組織(緩解期),觀察皮損中免疫組化CK20、S100A7、SP的錶達情況.結果 免疫組化圖像分析各組A值,髮病前期組、進展期組和緩解組CK20分彆為7683.80±6134.55、18305.04±13171.30、7257.53±4417.75.S100A7分彆為8789.05±6240.91、18058.01±16537.18、9295.65±9310.02.SP分另為3242.51±3775.41、9364.98±7596.64、2910.85±3349.46.進展期皮損組織中CK20與S100A7,SP錶達相對于髮病前期和緩解期明顯增加其差異均有統計學意義,P＜0.05.髮病前期組與緩解組其差異均無統計學意義,P＞0.05.CK20與S100A7、SP錶達呈正相關,相關繫數R分彆為0.779、0.876、P＜0.05.結論 銀屑病髮病與Merkel細胞數量的變化有一定的關繫.
목적 탐토불동병기은설병피손조직중CK20、S100A7、SP적표체급CK20여S100A7、SP표체적관계.방법 선택경과비정규치료,신구피손동시존재적환자19례,획취피손방적정상피부조직(발병전기)、피손조직(진전기)화피손수복후적병조피부조직(완해기),관찰피손중면역조화CK20、S100A7、SP적표체정황.결과 면역조화도상분석각조A치,발병전기조、진전기조화완해조CK20분별위7683.80±6134.55、18305.04±13171.30、7257.53±4417.75.S100A7분별위8789.05±6240.91、18058.01±16537.18、9295.65±9310.02.SP분령위3242.51±3775.41、9364.98±7596.64、2910.85±3349.46.진전기피손조직중CK20여S100A7,SP표체상대우발병전기화완해기명현증가기차이균유통계학의의,P＜0.05.발병전기조여완해조기차이균무통계학의의,P＞0.05.CK20여S100A7、SP표체정정상관,상관계수R분별위0.779、0.876、P＜0.05.결론 은설병발병여Merkel세포수량적변화유일정적관계.
Objective To explore the expressions of CK20, S100A7 and substance P (SP) in different stages of psoriatic lesions and their relationship. Methods A total of 19 patients, who had received irregular treatment for psoriasis and had both progressive and healed psoriatic lesions, were enrolled in this study. Skin tissue specimens were obtained from perilesional normal skin, progressive lesions and healed lesions of these patients and subjected to immunohistochemical analysis of expressions of CK20, S100A7 and SP. Results The relative expression level (absorbance value obtained from immunohistochemical analysis) was 7683.80 ± 6134.55,18305.04 ± 13171.30, 7257.53 ± 4417.75 for CK20, 8789.05 ± 6240.91, 18058.01 ± 16537.18, 9295.65 ±9310.02 for S100A7, 3242.51 ± 3775.41, 9364.98 ± 7596.64, 2910.85 ± 3349.46 for SP, respectively, in normal skin, progressive lesions and healed psoriatic lesions. A significant increase was observed in the expressions of CK20, S100A7 and SP in progressive lesions compared with normal skin and healed lesions, whereas no statistical difference was noted in those between normal skin and healed lesions (P ＞ 0.05 ). The expression of CK20 was positively correlated with that of S100A7 and SP (r = 0.779, 0.876, both P ＜ 0.05 ). Conclusion The pathogenesis of psoriasis seems to be associated with the changes in the number of Merkel cells.
