中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
9期
909-912
,共4页
星形细胞瘤%抗肿瘤联合化疗方案%顺铂%鬼臼噻吩甙%热疗%细胞增殖
星形細胞瘤%抗腫瘤聯閤化療方案%順鉑%鬼臼噻吩甙%熱療%細胞增殖
성형세포류%항종류연합화료방안%순박%귀구새분대%열료%세포증식
Astrocytoma%Antineoplastic combined with chemotherapy protocols%Cisplatin%Teniposide%Hyperthermia%Cell proliferation
目的 探讨热疗、化疗及两者序贯治疗对脑胶质瘤细胞增殖的影响.方法 体外传代培养的人星形细胞瘤系U-251细胞分别通过42℃、44℃高热环境和化疗药物顺铂、鬼臼噻吩甙在37℃恒温箱中孵育1 h;另取星形细胞瘤系U-251细胞同时行热疗和化疗联合应用1 h;再取星形细胞瘤系U-251细胞行序贯治疗,即先用1种处理方法,然后间隔1 h、4h,间隔期间在37℃恒温箱中孵育.治疗后采用MTT法测定肿瘤细胞增殖情况并进行统计学分析.结果 不同温度的热疗和不同种类的化疗药物对肿瘤细胞均可产生有效的杀伤作用,细胞增殖率明显下降.热化疗联合应用较单独应用能明显增加其抗肿瘤的效果,序贯治疗中可见44℃热疗+化疗组、44 ℃热疗后间隔1 h再行化疗组,其抗肿瘤的效果明显强于其他序贯处理组,差异有统计学意义(P＜0.05).结论 热化疗能有效杀伤肿瘤细胞,联合应用具有协同效应.两种治疗措施序贯治疗可见44 ℃高热预处理能提高肿瘤细胞对化疗药物的敏感性,但化疗药物的预处理效果相对较差,故该协同效应的机制可能是由于高热损伤了肿瘤细胞膜结构从而增加了药物的穿透所致.
目的 探討熱療、化療及兩者序貫治療對腦膠質瘤細胞增殖的影響.方法 體外傳代培養的人星形細胞瘤繫U-251細胞分彆通過42℃、44℃高熱環境和化療藥物順鉑、鬼臼噻吩甙在37℃恆溫箱中孵育1 h;另取星形細胞瘤繫U-251細胞同時行熱療和化療聯閤應用1 h;再取星形細胞瘤繫U-251細胞行序貫治療,即先用1種處理方法,然後間隔1 h、4h,間隔期間在37℃恆溫箱中孵育.治療後採用MTT法測定腫瘤細胞增殖情況併進行統計學分析.結果 不同溫度的熱療和不同種類的化療藥物對腫瘤細胞均可產生有效的殺傷作用,細胞增殖率明顯下降.熱化療聯閤應用較單獨應用能明顯增加其抗腫瘤的效果,序貫治療中可見44℃熱療+化療組、44 ℃熱療後間隔1 h再行化療組,其抗腫瘤的效果明顯彊于其他序貫處理組,差異有統計學意義(P＜0.05).結論 熱化療能有效殺傷腫瘤細胞,聯閤應用具有協同效應.兩種治療措施序貫治療可見44 ℃高熱預處理能提高腫瘤細胞對化療藥物的敏感性,但化療藥物的預處理效果相對較差,故該協同效應的機製可能是由于高熱損傷瞭腫瘤細胞膜結構從而增加瞭藥物的穿透所緻.
목적 탐토열료、화료급량자서관치료대뇌효질류세포증식적영향.방법 체외전대배양적인성형세포류계U-251세포분별통과42℃、44℃고열배경화화료약물순박、귀구새분대재37℃항온상중부육1 h;령취성형세포류계U-251세포동시행열료화화료연합응용1 h;재취성형세포류계U-251세포행서관치료,즉선용1충처리방법,연후간격1 h、4h,간격기간재37℃항온상중부육.치료후채용MTT법측정종류세포증식정황병진행통계학분석.결과 불동온도적열료화불동충류적화료약물대종류세포균가산생유효적살상작용,세포증식솔명현하강.열화료연합응용교단독응용능명현증가기항종류적효과,서관치료중가견44℃열료+화료조、44 ℃열료후간격1 h재행화료조,기항종류적효과명현강우기타서관처리조,차이유통계학의의(P＜0.05).결론 열화료능유효살상종류세포,연합응용구유협동효응.량충치료조시서관치료가견44 ℃고열예처리능제고종류세포대화료약물적민감성,단화료약물적예처리효과상대교차,고해협동효응적궤제가능시유우고열손상료종류세포막결구종이증가료약물적천투소치.
Objective To observe the influence of hyperthermia and/or chemotherapy on the cell proliferation of U-251 human astrocytoma cell line. Methods Some cells incubated in vitro were treated by hyperthermia (at a temperature of 42 ℃ and 44 ℃ for 1 h) and chemotherapy (with cis-platinum and teniposide at a temperature of 37 ℃ for 1 h), respectively; some cells were performed hyperthermia and chemotherapy simultaneously for 1 h; some cells were performed sequential therapy (with hyperthermia or chemotherapy first, and then with the other one 1 and 4 h after the former one at a temperature of 37 ℃). The cell proliferation was measured by MTT method and statistical analysis was performed. Results Hyperthermia or chemotherapy had cell-killing effects; hyperthermia at different temperatures or chemotherapy with cisplatin or teniposide could obviously decrease the cell proliferation rate. Simultaneous hyperthermia and chemotherapy could increase the anti-tumor effects as compared with hyperthermia or chemotherapy alone. The 2 kinds of sequential therapies ([performing hyperthermia at a temperature of 44 ℃ and chemotherapy 1 h after that at a temperature of 37 ℃ ], [performing hyperthermia at a temperature of 44 ℃ and chemotherapy at the same time]) had the strongest anti-tumor effects among all the kinds of sequential therapy, simultaneous hyperthermia chemotherapy have synergistic effects(P＜0.05). Conclusion Hyperthermia and/or chemotherapy have cell-killing effects;hyperthermia at a temperature of 44 ℃ can increase the susceptibility of chemotherapy, possibly resulting from destroying of cytomembrane and thus increasing the penetrability of cisplatin or teniposide.
