CAJ | 학술논문

目的探讨托吡酯(TPM)对小鼠汗腺部位乙酰胆碱酯酶(AChE)的表达和活性的影响.方法 2周龄的幼鼠每天经灌胃给予TPM(80 mg/kg),连续给药1个月后,进行汗腺部位AChE免疫组织化学和蛋白免疫印迹检测,观察汗腺部位AChE活性的变化.结果经TPM处理后汗腺AChE免疫荧光表达的吸光度(A)值为1.09±0.03,与对照组(0.98±0.09)比较差异无统计学意义(t=0.572,P＞0.05).蛋白免疫印迹表达结果亦差异无统计学意义(t=0.394,P＞0.05);汗腺组织匀浆后2组的AChE活性值分别为(1.42±0.38)μmol/mg和(1.37±0.42)μmol/mg,2组比较差异无统计学意义(t=0.746,P＞0.05).结论 TPM处理对汗腺部位的AChE免疫组织化学和蛋白免疫印迹表达及汗腺局部组织AChE活性无明显影响.
목적 탐토탁필지(TPM)대소서한선부위을선담감지매(AChE)적표체화활성적영향.방법 2주령적유서매천경관위급여TPM(80 mg/kg),련속급약1개월후,진행한선부위AChE면역조직화학화단백면역인적검측,관찰한선부위AChE활성적변화.결과 경TPM처리후한선AChE면역형광표체적흡광도(A)치위1.09±0.03,여대조조(0.98±0.09)비교차이무통계학의의(t=0.572,P＞0.05).단백면역인적표체결과역차이무통계학의의(t=0.394,P＞0.05);한선조직균장후2조적AChE활성치분별위(1.42±0.38)μmol/mg화(1.37±0.42)μmol/mg,2조비교차이무통계학의의(t=0.746,P＞0.05).결론 TPM처리대한선부위적AChE면역조직화학화단백면역인적표체급한선국부조직AChE활성무명현영향.
Objective To explore the correlationship between topiramate treatment and the expression and activity of acetylcholine esterase (AChE) in mouse footpad sweat gland.Methods Topiramate was given to two-weeks-old mice at 80 mg/kg for 1 month.By immunohistochemical staining and Western blot,the expression of AChE was analyzed in mouse footpad sweat gland in topiramte treatment group and non-treatment group.The AChE activity was determined by the method of Ellman with some modifications. Results By the immunostaining,AChE optical density value in the topiramate treatment group and control group was 1.09±0.03 and 0.98±0.09, respectively. There was no statistical difference (t=0.572,P>0.05). No significant difference of AChE expression was found between these two groups by Western blot (t =0.394,P>0.05). The AChE activity was (1.42 ±0.38) μmol/mg and (1.37±0.42) μmol/mg,respectively in treatment and control groups (t=0.746,P>0.05). Conclusions The expression and activity of AChE in mouse footpad sweat gland are not affected by topiramate treatment.