中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2009年
8期
524-527
,共4页
王玉苹%宋秀豹%洪其生%李卫
王玉蘋%宋秀豹%洪其生%李衛
왕옥평%송수표%홍기생%리위
脑损伤%磁疗%凋亡
腦損傷%磁療%凋亡
뇌손상%자료%조망
Brain injury%Magnetotherapy%Apoptosis
目的 研究磁疗对大鼠创伤性脑组织损伤后神经功能的保护作用及其可能机制.方法 制作20只大鼠创伤性腩组织损伤模型,随机分为磁疗组和非磁疗组.磁疗组头部创面区接受磁疗,磁场强度为表面强度0.15 T,旋转时强度0.1 T,转速为3000转/min,每天治疗2次,每次20 min,共10 d.非磁疗组不接受磁疗.伤后第11天测定大鼠左后肢腓肠肌的收缩力,随后取脑组织标本,免疫组化方法观察损伤灶周边分化抗原簇4阳性(CD4+)、分化抗原簇8 阳性(CD8+)细胞浸润及细胞凋亡情况,分析各指标间的关系.结果 伤后第11天,磁疗组大鼠损伤灶周围的CD4+、CD8+细胞浸润及细胞凋亡数量明显少于非磁疗组,而磁疗组左后肢腓肠肌的收缩力却明显强于非磁疗组.结论 磁疗可能通过抑制脑组织损伤灶局部的淋巴细胞浸润及神经细胞凋亡,减轻脑组织的进一步损伤,保护了神经功能.
目的 研究磁療對大鼠創傷性腦組織損傷後神經功能的保護作用及其可能機製.方法 製作20隻大鼠創傷性腩組織損傷模型,隨機分為磁療組和非磁療組.磁療組頭部創麵區接受磁療,磁場彊度為錶麵彊度0.15 T,鏇轉時彊度0.1 T,轉速為3000轉/min,每天治療2次,每次20 min,共10 d.非磁療組不接受磁療.傷後第11天測定大鼠左後肢腓腸肌的收縮力,隨後取腦組織標本,免疫組化方法觀察損傷竈週邊分化抗原簇4暘性(CD4+)、分化抗原簇8 暘性(CD8+)細胞浸潤及細胞凋亡情況,分析各指標間的關繫.結果 傷後第11天,磁療組大鼠損傷竈週圍的CD4+、CD8+細胞浸潤及細胞凋亡數量明顯少于非磁療組,而磁療組左後肢腓腸肌的收縮力卻明顯彊于非磁療組.結論 磁療可能通過抑製腦組織損傷竈跼部的淋巴細胞浸潤及神經細胞凋亡,減輕腦組織的進一步損傷,保護瞭神經功能.
목적 연구자료대대서창상성뇌조직손상후신경공능적보호작용급기가능궤제.방법 제작20지대서창상성남조직손상모형,수궤분위자료조화비자료조.자료조두부창면구접수자료,자장강도위표면강도0.15 T,선전시강도0.1 T,전속위3000전/min,매천치료2차,매차20 min,공10 d.비자료조불접수자료.상후제11천측정대서좌후지비장기적수축력,수후취뇌조직표본,면역조화방법관찰손상조주변분화항원족4양성(CD4+)、분화항원족8 양성(CD8+)세포침윤급세포조망정황,분석각지표간적관계.결과 상후제11천,자료조대서손상조주위적CD4+、CD8+세포침윤급세포조망수량명현소우비자료조,이자료조좌후지비장기적수축력각명현강우비자료조.결론 자료가능통과억제뇌조직손상조국부적림파세포침윤급신경세포조망,감경뇌조직적진일보손상,보호료신경공능.
Objective To study the neuro-protective effects of magnetotherapy after traumatic brain injury of rats. Methods Traumatic brain injury models were created in 20 rats and divided randomly into magnetotherapy and non-magnetotherapy groups. The rats in magnetotherapy group received magnetotherapy on the cranial surface of traumatized region twice daily, each for 20 min, over 10 d. The intensity of magnetic field was O. 15 T at the surface,and 0.1 T during spinning at 3000 rpm. The contraction strength of gastrocnemius of each rat's left hind limb was measured on the 11 th d after trauma. The rats were then sacrificed and brain tissue samples were taken. The infiltra-tions of CD4 + and CD8 + cells and apnptosis in the area around the injury lesion were observed with immunohisto-chemical methods. The relationship among the indexes was analyzed. Results At the 11th d after brain injury, the quantities of CD4 + and CD8 + cells and apoptotic cells in magnetotherapy group were all obviously less than those in non-magnetotherapy group. Average contraction strength of gastrocnemius in magnetotherapy group was significantly stronger than that in non-magnetotherapy group. Conclusions Magnetotherapy can relieve the secondary effects of brain injury and induce neuro-protection effects. This may involve decreasing the local infiltrations of CD4 + and CD8 + and apoptotic cells in the area around the injury lesion.
