CAJ | 학술논문

利用普通小麦(Triticum aesttvum L.)"小偃6号"与黑麦(Secale cereale L.)品种"德国白粒"杂交,选育出"小偃6号"类型带有黑麦性状的种质材料.应用总基因组原位杂交(GISH)进行检测,在8份材料中探测到黑麦染色质的存在,其中附加系3个,代换系1个,易位系4个;进一步用荧光绿标记探针pScll9.2及荧光红标记探针pAsl的双色荧光原位杂交(FISH)技术,对其中部分品系的染色体组成进行分析鉴定,结果表明:易位系BCll6-1是1RS/1BL小麦/黑麦易位系,BCl52-l是涉及一条lB染色体的1RS/1BL易位系,代换系BC97-2是2R(2D)二体代换系;附加系BCl22-3附加了一条6R黑麦染色体,一条6B染色体的长臂缺失.同时,对连续的总基因组原位杂交和双色荧光原位杂交技术在小麦育种中的应用进行了讨论.
이용보통소맥(Triticum aesttvum L.)"소언6호"여흑맥(Secale cereale L.)품충"덕국백립"잡교,선육출"소언6호"류형대유흑맥성상적충질재료.응용총기인조원위잡교(GISH)진행검측,재8빈재료중탐측도흑맥염색질적존재,기중부가계3개,대환계1개,역위계4개;진일보용형광록표기탐침pScll9.2급형광홍표기탐침pAsl적쌍색형광원위잡교(FISH)기술,대기중부분품계적염색체조성진행분석감정,결과표명:역위계BCll6-1시1RS/1BL소맥/흑맥역위계,BCl52-l시섭급일조lB염색체적1RS/1BL역위계,대환계BC97-2시2R(2D)이체대환계;부가계BCl22-3부가료일조6R흑맥염색체,일조6B염색체적장비결실.동시,대련속적총기인조원위잡교화쌍색형광원위잡교기술재소맥육충중적응용진행료토론.
A set of wheat germplasms with rye's characters was obtained by crossing the Triticum aestivum L. cv. "Xiaoyan No.6" and Secale cereale L. cv. "German White". Eight lines were found carrying rye chromosomes or segments by genomic in situ hybridization (GISH), including three addition lines, one substitution line and four translocation lines. Two-color fluorescent in situ hybridization (FISH) was used to identify the genome composition and individual chromosome of the hybrids using pSc119.2 labled with fluorogreen and pAs1labled with fluorored as probes. The partial results showed that BC116-1 is a wheatrye 1RS/1BL translocation line; BC152 is a monosomic 1RS/1BL translocation line; BC97-2 is a disomic substitution line with a pair of 2D chromosomes substituted by 2R; BC122-3 is a 6R monosomic addition line with one of the long arm of 6B deletion. The application of sequential GISH and two-color FISH in wheat breeding is discussed.