CAJ | 학술논문

背景:应用抖笼换能器法、阈上、阈下剂量戊巴比妥钠睡眠时间的实验法,探讨橙皮水提取物对昆明种小鼠睡眠和自发活动的影响.目的:观察橙皮水提取物对昆明种小鼠睡眠和自发活动的影响.设计:随机对照实验观察.单位:赣南医学院病理教研室、生理学教研室及药理教研室.材料:实验于2005-01/02在赣南医学院科研中心实验室完成.选择健康成年昆明种清洁级小鼠90只,每种实验方法30只,随机分为3组,对照组,小剂量给药组,大剂量给药组;每组10只.方法:①采用抖笼换能器法记录小鼠自发活动情况,小波(波幅＜5 mm)为小鼠轻度活动(舐毛、瘙痒)的波形;中波(波幅5～10 mm)为小鼠中度活动(走动、立起、爬上、爬下)的波形;大波(波幅＞10 mm以上)为小鼠强度活动(跑动、上串下跳)的波形.小剂量给药组、大剂量给药组分别腹腔注射橙皮水提取物5 mg/g、10 mg/g和对照组注射等量生理盐水,15 min后放回吊笼中,稳定3 min后记录15s内小鼠活动波形.②橙皮水提取物对小鼠阈上或阈下剂量戊巴比妥钠睡眠时间的实验法:小剂量给药组、大剂量给药组分别腹腔注射橙皮水提取物5 mg/g、10 mg/g,对照组注射等量生理盐水;15 min后,3组均腹腔注射阈上剂量的戊巴比妥钠(2.5g/L)0.02 mL/g,或阈下剂量的戊巴比妥钠(2.5 g/L)0.01 mL/g,比较给药组与对照组动物的入睡个数、入睡时间和睡眠持续时间.主要观察指标:①小鼠自发活动次数.②阈上剂量戊巴比妥钠小鼠睡眠时间.③阈下剂量戊巴比妥钠小鼠睡眠时间.结果:各实验组小鼠无脱失情况,全部进入结果分析.①橙皮水提取物小剂量给药组,大剂量给药组使中波、大波出现次数显著减少,对小鼠自发活动具有明显的抑制作用,[(90.5±14.7),(58.3±12.5),(45.6±10.1),t=2.341～3.215;P＜0.05或P＜0.01].②橙皮水提取物小剂量给药组,大剂量给药组能显著加速阈上剂量戊巴比妥钠的入睡时间和延长戊巴比妥钠的睡眠时间,差异有显著性意义.③橙皮水提取物小剂量给药组,大剂量给药组能显著加速阈下剂量戊巴比妥钠的入睡时间和延长戊巴比妥钠的睡眠时间,差异有非常显著性意义.结论:橙皮水提取物具有明显的延长昆明种小鼠睡眠时间和抑制小鼠自发性活动.与戊巴比妥钠有协同的中枢抑制作用. 揹景:應用抖籠換能器法、閾上、閾下劑量戊巴比妥鈉睡眠時間的實驗法,探討橙皮水提取物對昆明種小鼠睡眠和自髮活動的影響.目的:觀察橙皮水提取物對昆明種小鼠睡眠和自髮活動的影響.設計:隨機對照實驗觀察.單位:贛南醫學院病理教研室、生理學教研室及藥理教研室.材料:實驗于2005-01/02在贛南醫學院科研中心實驗室完成.選擇健康成年昆明種清潔級小鼠90隻,每種實驗方法30隻,隨機分為3組,對照組,小劑量給藥組,大劑量給藥組;每組10隻.方法:①採用抖籠換能器法記錄小鼠自髮活動情況,小波(波幅＜5 mm)為小鼠輕度活動(舐毛、瘙癢)的波形;中波(波幅5～10 mm)為小鼠中度活動(走動、立起、爬上、爬下)的波形;大波(波幅＞10 mm以上)為小鼠彊度活動(跑動、上串下跳)的波形.小劑量給藥組、大劑量給藥組分彆腹腔註射橙皮水提取物5 mg/g、10 mg/g和對照組註射等量生理鹽水,15 min後放迴弔籠中,穩定3 min後記錄15s內小鼠活動波形.②橙皮水提取物對小鼠閾上或閾下劑量戊巴比妥鈉睡眠時間的實驗法:小劑量給藥組、大劑量給藥組分彆腹腔註射橙皮水提取物5 mg/g、10 mg/g,對照組註射等量生理鹽水;15 min後,3組均腹腔註射閾上劑量的戊巴比妥鈉(2.5g/L)0.02 mL/g,或閾下劑量的戊巴比妥鈉(2.5 g/L)0.01 mL/g,比較給藥組與對照組動物的入睡箇數、入睡時間和睡眠持續時間.主要觀察指標:①小鼠自髮活動次數.②閾上劑量戊巴比妥鈉小鼠睡眠時間.③閾下劑量戊巴比妥鈉小鼠睡眠時間.結果:各實驗組小鼠無脫失情況,全部進入結果分析.①橙皮水提取物小劑量給藥組,大劑量給藥組使中波、大波齣現次數顯著減少,對小鼠自髮活動具有明顯的抑製作用,[(90.5±14.7),(58.3±12.5),(45.6±10.1),t=2.341～3.215;P＜0.05或P＜0.01].②橙皮水提取物小劑量給藥組,大劑量給藥組能顯著加速閾上劑量戊巴比妥鈉的入睡時間和延長戊巴比妥鈉的睡眠時間,差異有顯著性意義.③橙皮水提取物小劑量給藥組,大劑量給藥組能顯著加速閾下劑量戊巴比妥鈉的入睡時間和延長戊巴比妥鈉的睡眠時間,差異有非常顯著性意義.結論:橙皮水提取物具有明顯的延長昆明種小鼠睡眠時間和抑製小鼠自髮性活動.與戊巴比妥鈉有協同的中樞抑製作用.
배경:응용두롱환능기법、역상、역하제량무파비타납수면시간적실험법,탐토등피수제취물대곤명충소서수면화자발활동적영향.목적:관찰등피수제취물대곤명충소서수면화자발활동적영향.설계:수궤대조실험관찰.단위:공남의학원병리교연실、생이학교연실급약리교연실.재료:실험우2005-01/02재공남의학원과연중심실험실완성.선택건강성년곤명충청길급소서90지,매충실험방법30지,수궤분위3조,대조조,소제량급약조,대제량급약조;매조10지.방법:①채용두롱환능기법기록소서자발활동정황,소파(파폭＜5 mm)위소서경도활동(지모、소양)적파형;중파(파폭5～10 mm)위소서중도활동(주동、립기、파상、파하)적파형;대파(파폭＞10 mm이상)위소서강도활동(포동、상천하도)적파형.소제량급약조、대제량급약조분별복강주사등피수제취물5 mg/g、10 mg/g화대조조주사등량생리염수,15 min후방회조롱중,은정3 min후기록15s내소서활동파형.②등피수제취물대소서역상혹역하제량무파비타납수면시간적실험법:소제량급약조、대제량급약조분별복강주사등피수제취물5 mg/g、10 mg/g,대조조주사등량생리염수;15 min후,3조균복강주사역상제량적무파비타납(2.5g/L)0.02 mL/g,혹역하제량적무파비타납(2.5 g/L)0.01 mL/g,비교급약조여대조조동물적입수개수、입수시간화수면지속시간.주요관찰지표:①소서자발활동차수.②역상제량무파비타납소서수면시간.③역하제량무파비타납소서수면시간.결과:각실험조소서무탈실정황,전부진입결과분석.①등피수제취물소제량급약조,대제량급약조사중파、대파출현차수현저감소,대소서자발활동구유명현적억제작용,[(90.5±14.7),(58.3±12.5),(45.6±10.1),t=2.341～3.215;P＜0.05혹P＜0.01].②등피수제취물소제량급약조,대제량급약조능현저가속역상제량무파비타납적입수시간화연장무파비타납적수면시간,차이유현저성의의.③등피수제취물소제량급약조,대제량급약조능현저가속역하제량무파비타납적입수시간화연장무파비타납적수면시간,차이유비상현저성의의.결론:등피수제취물구유명현적연장곤명충소서수면시간화억제소서자발성활동.여무파비타납유협동적중추억제작용.
BACKGROUND: Effects of Pericarpium aurantiin water extract on sleeping and spontaneous activity in Kunming mice has been investigated in three experiments, that is, the experiments of sleeping time due to supraliminal and subliminal dosage of sodium pentobarbital, and shaking cage for energy transducer, respectively.OBJECTIVE: To observe the influence of Pericarpium aurantiin water extract on sleeping and spontaneous activity of Kunming mice.DESIGN:Randomized controlled trial.SETTING: The Staff Rooms of Pathology, Physiology and Pharmacology in Gannan Medical College.MATERIALS: The experiment was conducted in the Central Laboratory of Science Research, Gannan Medical College from January through February 2005. Ninety adult Kunming mice, grade of clearness, were enrolled and randomly divided into three groups with 30 mice for each experiment:10 in control group, 10 in large-dosage group and 10 in small-dosage group.METHODS: ① In energy transforming trial, spontaneous activity of mice was recorded, small wave with wave amplitude ＜ 5 mm was considered as the waveform of gentle activity in mice, such as fur licking and tickling; middle wave with wave amplitude of 5-10 mm as the waveform of moderate activity, such as walking, standing, climbing up and down;large wave with wave amplitude ＞ 10 mm as the waveform of intensive activity, such as running, jumping up and down. Mice in the small-dosage group, large-dosage group and control group were respectively given intraperitoneal injection of 5 mg/g, 10 mg/g Pericarpium aurantiin water extract and equal volume of normal saline, and placed into the cage after 15 minutes. Then, 3 minutes later, waveforms of mice within 15 s was recorded. ② In the experiments on duration of sleep in mice due to supraliminal or subliminal dosage sodium pentobarbital, 0.02 mL/g or 0.01 mL/g sodium pentobarbital (2.5 g/L) were administrated intraperitoneally into mice in the small-dosage, large-dosage and control group 15 minutes after intraperitoneal injection of 5 mg/g, 10 mg/g Pericarpium aurantiin water extract and equal volume of normal saline were given to the three groups, respectively. Subsequently, comparisons of the number of sleeping mice, time to falling asleep, and sleeping time were carried out among the three groups.MAIN OUTCOME MEASURFS: ① Times of spontaneous activity, ②Sleeping time due to supraliminal dosage of sodium pentobarbital, ③ Duration of sleep due to subliminal dosage of sodium pentobarbital.RESULTS: All the mice were involved in the result analysis without loss.① There were fewer middle and large waves in the small-dosage and largedosage groups as compared with that in the control group (90.5±14.7,58.3±12.5, 45.6±10.1, t=2.341-3.215; P ＜0.05 or P ＜0.01), indicating that Pericarpium aurantiin water extract can evidently inhibit spontaneous activities in mice. ② In comparison with the control group, the time to falling asleep and the duration of sleep due to supraliminal dosage of sodium pentobarbital were shortened and prolonged significantly, respectively,in the small-dosage and large-dosage groups. ③ In comparison with the control group, the time to falling asleep and duration of sleep due to subliminal dosage of sodium pentobarbital were shortened and prolonged significantly, respectively, in the small-dosage and large-dosage groups.CONCLUSION: Pericarpium aurantiin water extract can prolong the duration of sleep and inhibit the spontaneous activities of Kunming mice,which is in cooperation with sodium pentobarbital in central inhibition.