中国医院药学杂志
中國醫院藥學雜誌
중국의원약학잡지
CHINESE JOURNAL OF HOSPITAL PHARMACY
2007年
2期
191-193
,共3页
聂鑫%罗佳波%吕本强%邢学峰
聶鑫%囉佳波%呂本彊%邢學峰
섭흠%라가파%려본강%형학봉
黄芩苷%黄芩素%汉黄芩苷%汉黄芩素%高效液相色谱法
黃芩苷%黃芩素%漢黃芩苷%漢黃芩素%高效液相色譜法
황금감%황금소%한황금감%한황금소%고효액상색보법
目的:建立黄芩苷粗品中黄芩苷、黄芩素、汉黄苓苷和汉黄苓素含量的高效液相色谱(HPLC)测定方法,以便控制其质量.方法:固定相用Agilent ZORBAX SB-C18柱(5 μm,4.6 mm×250 mm),苷流动相为甲醇-水-冰醋酸(49∶51∶0.2),苷元流动相为甲醇-水-冰醋酸(61∶39∶0.2),检测波长为275 nm.结果:黄芩苷、黄芩素、汉黄芩苷和汉黄芩素线性范围分别是0.102～1.020 μg(r=0.999 7),0.051～0.510 μg(r=0.999 4),0.01～0.51 μg(r=0.999 5),0.02～0.20 μg(r=0.999 9);平均回收率(n=5)分别为99.12%(RSD为1.55%),101.60%(RSD为2.67%),102.27%(RSD为1.92%)和98.51%(RSD为3.13%).结论:本方法简便、准确,可用于黄芩苷粗品的质量控制.
目的:建立黃芩苷粗品中黃芩苷、黃芩素、漢黃苓苷和漢黃苓素含量的高效液相色譜(HPLC)測定方法,以便控製其質量.方法:固定相用Agilent ZORBAX SB-C18柱(5 μm,4.6 mm×250 mm),苷流動相為甲醇-水-冰醋痠(49∶51∶0.2),苷元流動相為甲醇-水-冰醋痠(61∶39∶0.2),檢測波長為275 nm.結果:黃芩苷、黃芩素、漢黃芩苷和漢黃芩素線性範圍分彆是0.102～1.020 μg(r=0.999 7),0.051～0.510 μg(r=0.999 4),0.01～0.51 μg(r=0.999 5),0.02～0.20 μg(r=0.999 9);平均迴收率(n=5)分彆為99.12%(RSD為1.55%),101.60%(RSD為2.67%),102.27%(RSD為1.92%)和98.51%(RSD為3.13%).結論:本方法簡便、準確,可用于黃芩苷粗品的質量控製.
목적:건립황금감조품중황금감、황금소、한황령감화한황령소함량적고효액상색보(HPLC)측정방법,이편공제기질량.방법:고정상용Agilent ZORBAX SB-C18주(5 μm,4.6 mm×250 mm),감류동상위갑순-수-빙작산(49∶51∶0.2),감원류동상위갑순-수-빙작산(61∶39∶0.2),검측파장위275 nm.결과:황금감、황금소、한황금감화한황금소선성범위분별시0.102～1.020 μg(r=0.999 7),0.051～0.510 μg(r=0.999 4),0.01～0.51 μg(r=0.999 5),0.02～0.20 μg(r=0.999 9);평균회수솔(n=5)분별위99.12%(RSD위1.55%),101.60%(RSD위2.67%),102.27%(RSD위1.92%)화98.51%(RSD위3.13%).결론:본방법간편、준학,가용우황금감조품적질량공제.
