CAJ | 학술논문

目的探讨Nd:YAP激光不同方式照射活体大鼠肝脏对组织的不同影响.方法大鼠15只,按照射方式分为非接触式、接触式、插入式3大组,每组5只鼠.每组分别以功率为3.0、3.5、4.0、4.5和5.0 W,照射时间4 s,光斑直径为0.5 mm的Nd:YAP激光照射大鼠中叶、右侧叶肝脏.每脏叶照射3～6点,点间间隔＞0.5cm.照射后肉眼观察肝脏变化,并于照射处取材,常规石蜡切片,HE染色,光镜下观察.结果各组以功率3.0～3.5 W的Nd:YAP激光照射,以凝固性变化为主,随着功率增加除凝固效应外还逐渐表现出气化效应,组织穿透深度也由约0.5 mm逐渐增加至约2.5 mm.与相同功率和作用时间非接触式照射比较,接触式照射和插入式照射组织出现更多的缺损和更深的穿透深度.非接触式照射功率较高时出现组织爆破现象,接触式照射和插入式照射组织反应均平稳,无爆破现象,插入式照射较易伤及周围肝脏组织.结论非接触式照射、接触式照射和插入式照射是Nd:YAP激光照射肿物的可行性方法,可根据肿瘤组织厚度适当选择非接触式照射、接触式照射和插入式照射方式进行照射治疗,但应正确掌握照射功率避免引起照射处周围组织的损伤.
목적 탐토Nd:YAP격광불동방식조사활체대서간장대조직적불동영향.방법 대서15지,안조사방식분위비접촉식、접촉식、삽입식3대조,매조5지서.매조분별이공솔위3.0、3.5、4.0、4.5화5.0 W,조사시간4 s,광반직경위0.5 mm적Nd:YAP격광조사대서중협、우측협간장.매장협조사3～6점,점간간격＞0.5cm.조사후육안관찰간장변화,병우조사처취재,상규석사절편,HE염색,광경하관찰.결과 각조이공솔3.0～3.5 W적Nd:YAP격광조사,이응고성변화위주,수착공솔증가제응고효응외환축점표현출기화효응,조직천투심도야유약0.5 mm축점증가지약2.5 mm.여상동공솔화작용시간비접촉식조사비교,접촉식조사화삽입식조사조직출현경다적결손화경심적천투심도.비접촉식조사공솔교고시출현조직폭파현상,접촉식조사화삽입식조사조직반응균평은,무폭파현상,삽입식조사교역상급주위간장조직.결론 비접촉식조사、접촉식조사화삽입식조사시Nd:YAP격광조사종물적가행성방법,가근거종류조직후도괄당선택비접촉식조사、접촉식조사화삽입식조사방식진행조사치료,단응정학장악조사공솔피면인기조사처주위조직적손상.
To explore the influence of different irradiation methods on liver ire vivo rats by Nd: YAP laser.Methods Fifteen rats were divided into three groups according to the operation methods, there were no-contact, contact and insertion irradiation groups, respectively, five rats in each group. Nd: YAP laser was used to irradiate liver middle and right lobes with light spot of 0.5 mm diameter, and the irradiation power was 3. 0, 3. 5, 4. 0, 4. 5 and 5. 0 W, respectively, in varous groups, the working time was 4 s. Every hepatic lobe was irrdiated with 3 to 6 light spots with interval above 0. 5 cm. After that the morphology and pathological changes of cases under light microscope were observed.Results The tissues were mainly coagulated in 3. 0-3. 5 W by all three methods. As increasing in power it aslo demonstrated the gasification , and the penetrating depth was gradually increased from about 0. 5 mm to 2. 5 mm. Compared with non-contact irradiation, tissues with contact irradiation and insertion irradiation had more damaged and deeper penetration depth at the same power and action time. The tissues blasted when exposure at a higher power with non-contact irradiation while the rest were stable in the used power dose of the experiment. The insertion irradiation seemed easy to damage tissues around.Conclusions Nd: YAP laser is a feasible method to treat tumors which can choose non-contact irradiation, contact irradiation or insertion irradiation with the power of 3.0-4. 5, 3-5, and 3-4 W, respectively, according to the thickness of tissues, but the operator should know well the power action thus avoiding damage to the surrounding tissues.