CAJ | 학술논문

参考GenBank中多个BVDV毒株基因组序列,利用生物软件primer5.0设计两对引物,扩增出BVDV牦牛株 P20基因和P14基因.结果表明,克隆得到的P20基因序列为504 bp,与GenBank上发表的BVDV P20大小一致,P14基因序列为312 bp,与GenBank上发表的BVDV毒株比较有6个插入碱基.核苷酸序列的同源性及系统发生分析的结果表明,牦牛(Yak)株属于BVDV-1.
삼고GenBank중다개BVDV독주기인조서렬,이용생물연건primer5.0설계량대인물,확증출BVDV모우주 P20기인화P14기인.결과표명,극륭득도적P20기인서렬위504 bp,여GenBank상발표적BVDV P20대소일치,P14기인서렬위312 bp,여GenBank상발표적BVDV독주비교유6개삽입감기.핵감산서렬적동원성급계통발생분석적결과표명,모우(Yak)주속우BVDV-1.
According to the sequence of BVDV strains published by Genbank, two set of primers were designed by primer premier 5.0 and used to amplify by PCR .The p20 and p14 gene of BVDV in Yak were firstly cloned. Two specific 504bp and 312bp DNA product were amplified .The result confirms that the length of p20 gene is accordant with other BVDV strains published by Genbank, and the Yak strain possess six base insertion in the p14 gene region. The Yak strain of BVDV belongs to genotype I by nucleotide homololgy analyses and the phylogenetic analyses.