四川大学学报(自然科学版)
四川大學學報(自然科學版)
사천대학학보(자연과학판)
JOURNAL OF SICHUAN UNIVERSITY
2009年
6期
1805-1810
,共6页
冯英阳%刘亚刚%于吉锋%常文棣%胡炳峰%王文伯
馮英暘%劉亞剛%于吉鋒%常文棣%鬍炳峰%王文伯
풍영양%류아강%우길봉%상문체%호병봉%왕문백
牦牛%牛病毒性腹泻病毒%P20基因和P14基因%克隆%序列分析
牦牛%牛病毒性腹瀉病毒%P20基因和P14基因%剋隆%序列分析
모우%우병독성복사병독%P20기인화P14기인%극륭%서렬분석
Yak%BVDV%p20 and p14 gene%cloning%sequence analysis
参考GenBank中多个BVDV毒株基因组序列,利用生物软件primer5.0设计两对引物,扩增出BVDV牦牛株 P20基因和P14基因.结果表明,克隆得到的P20基因序列为504 bp,与GenBank上发表的BVDV P20大小一致,P14基因序列为312 bp,与GenBank上发表的BVDV毒株比较有6个插入碱基.核苷酸序列的同源性及系统发生分析的结果表明,牦牛(Yak)株属于BVDV-1.
參攷GenBank中多箇BVDV毒株基因組序列,利用生物軟件primer5.0設計兩對引物,擴增齣BVDV牦牛株 P20基因和P14基因.結果錶明,剋隆得到的P20基因序列為504 bp,與GenBank上髮錶的BVDV P20大小一緻,P14基因序列為312 bp,與GenBank上髮錶的BVDV毒株比較有6箇插入堿基.覈苷痠序列的同源性及繫統髮生分析的結果錶明,牦牛(Yak)株屬于BVDV-1.
삼고GenBank중다개BVDV독주기인조서렬,이용생물연건primer5.0설계량대인물,확증출BVDV모우주 P20기인화P14기인.결과표명,극륭득도적P20기인서렬위504 bp,여GenBank상발표적BVDV P20대소일치,P14기인서렬위312 bp,여GenBank상발표적BVDV독주비교유6개삽입감기.핵감산서렬적동원성급계통발생분석적결과표명,모우(Yak)주속우BVDV-1.
According to the sequence of BVDV strains published by Genbank, two set of primers were designed by primer premier 5.0 and used to amplify by PCR .The p20 and p14 gene of BVDV in Yak were firstly cloned. Two specific 504bp and 312bp DNA product were amplified .The result confirms that the length of p20 gene is accordant with other BVDV strains published by Genbank, and the Yak strain possess six base insertion in the p14 gene region. The Yak strain of BVDV belongs to genotype I by nucleotide homololgy analyses and the phylogenetic analyses.