CAJ | 학술논문

环氧化物水解酶(EH)是一类能催化外消旋环氧化物水解生成有光学活性的环氧化物和二醇的酶,应用前景广阔.自然界筛选到的微生物往往存在产酶活力低、对映体选择性不高等问题.近年来,基因工程技术的发展及其在微生物环氧化物水解酶中的应用,改善了酶的催化特性,为酶的工业化应用提供了条件.该文简单介绍了微生物环氧化物水解酶的催化反应机制和快速检测方法,详细介绍了环氧化物水解酶基因工程方面的研究进展.
배양화물수해매(EH)시일류능최화외소선배양화물수해생성유광학활성적배양화물화이순적매,응용전경엄활.자연계사선도적미생물왕왕존재산매활력저、대영체선택성불고등문제.근년래,기인공정기술적발전급기재미생물배양화물수해매중적응용,개선료매적최화특성,위매적공업화응용제공료조건.해문간단개소료미생물배양화물수해매적최화반응궤제화쾌속검측방법,상세개소료배양화물수해매기인공정방면적연구진전.
Epoxide hydrolase, which could transform racemic epoxide into enantiomeric vicinal diol and entantioepoxide, had a broad prospect for its application. Some high - throughput assay methods had been established and were used to screen wildtype microorganism.However, the low enzyme activity and enantioselectivity were existed in wildtype microorganism. Recently, with the development of gene engineering and its application to microbial epoxide hydrolase, catalytic properties were improved. Reaction mechanism of microbial epoxide hydrolase and high - throughput assay methods were briefly reviewed in this paper, the research of gene engineering in microbial expoxide hydrolase were described in detail.