CAJ | 학술논문

采用大孔吸附树脂分离纯化白屈菜中白屈莱红碱,并对白屈菜红碱的抑菌效果进行了研究.结果表明,AL-2型大孔吸附树脂对白屈菜红碱具有良好的吸附和解吸性能,效果最佳,适宜的分离条件为吸附温度30℃,上样液浓度为0.9 mg/mL,上样流速为1.0 mL/min,样品洗脱乙醇体积分数为30%.以1.5mL/min速度洗脱时,洗脱液量为4BV为洗脱终点;纯化后的白屈菜红碱对甜瓜枯萎病病菌、西瓜枯萎病病菌、茄子黄萎病病菌、黄瓜枯萎病病菌、辣椒炭疽病病菌的茼丝生长均其一定抑制作用.
채용대공흡부수지분리순화백굴채중백굴래홍감,병대백굴채홍감적억균효과진행료연구.결과표명,AL-2형대공흡부수지대백굴채홍감구유량호적흡부화해흡성능,효과최가,괄의적분리조건위흡부온도30℃,상양액농도위0.9 mg/mL,상양류속위1.0 mL/min,양품세탈을순체적분수위30%.이1.5mL/min속도세탈시,세탈액량위4BV위세탈종점;순화후적백굴채홍감대첨과고위병병균、서과고위병병균、가자황위병병균、황과고위병병균、랄초탄저병병균적동사생장균기일정억제작용.
The chelerythrine from Chelidonium majus by macroporous resin adsorbent,and its anti-fungal activity was observed. The result showed that the macroporous resin adsorbent AL-2 was the optimal absorbent material. The optimum con-ditions of adsorption and desorption were as follows: adsorption temperature 30 C, sample concentration 0.9 mg/mL, adsorp-tion speed 1.0 mL/min, and the volume of 30%(V/V) ethanol 4 BV as eluant with desorption speed 1.5 mL/min. The purified chelerythrine showed a certain inhibitory effect on Fusarium oxysporum f. Sp. Metonis, Fusarium oxysporum f. Sp. Niveum, Verticillium dahliae, Fusarium oxysponun f.sp. Cucumerinum, Vermicularia capsici.