CAJ | 학술논문

在香蕉果肉可溶态Ca含量分析过程中,引入体外消化模型进行预处理,采用AA700型火焰原子吸收分光光度计测量Ca含量.结果表明:(1)八成熟香蕉果肉中Ca含量为1.479 mg·g~(-1),平行分析实验结果RSD为4.12%;(2)八成熟香蕉果肉经过体外消化模型处理后,可溶态和不溶态Ca含量分别为1.108和0.412 mg·g~(-1),Ca溶解率和残留率分别为74.9%和27.8%,Ca主要以可溶态形式存在;(3)Ca溶解率和残留率平行实验结果的RSD分别为2.56%和9.10%,说明体外消化模型的引入并没有对实验方法精密度产生显著影响,且溶解率和残留率之和与理论值的误差仅为2.7%,进一步说明在食品微量元素形态分离分析过程中引人体外消化模型可行;(4)ca测试方法的精密度和回收实验表明,样品回收率为99.2%,RSD为0.11%(n=9).
재향초과육가용태Ca함량분석과정중,인입체외소화모형진행예처리,채용AA700형화염원자흡수분광광도계측량Ca함량.결과표명:(1)팔성숙향초과육중Ca함량위1.479 mg·g~(-1),평행분석실험결과RSD위4.12%;(2)팔성숙향초과육경과체외소화모형처리후,가용태화불용태Ca함량분별위1.108화0.412 mg·g~(-1),Ca용해솔화잔류솔분별위74.9%화27.8%,Ca주요이가용태형식존재;(3)Ca용해솔화잔류솔평행실험결과적RSD분별위2.56%화9.10%,설명체외소화모형적인입병몰유대실험방법정밀도산생현저영향,차용해솔화잔류솔지화여이론치적오차부위2.7%,진일보설명재식품미량원소형태분리분석과정중인인체외소화모형가행;(4)ca측시방법적정밀도화회수실험표명,양품회수솔위99.2%,RSD위0.11%(n=9).
In the present paper, soluble Ca content in banana pulp was analyzed with pretreatment of digestion model in vitro. All the samples were digested by strong acid (perchloric acid and nitric acid with 1:4 ratio) and determined by AAT00 flame a-tomic absorption spectrophotometry (FAAS). Meanwhile, the reproducibility of the analytical method was investigated. The re-sults showed that (1) Content of Ca was 1.479 mg ·g~(-1)·DW in eighty percent maturity banana pulp. And the relative standard deviation was 4.12%. (2) Contents of Ca in the soluble and insoluble fractions were 1. 108 and 0. 412 mg·g~(-1)·DW, respec-tively in eighty percent maturity banana pulp with pretreatment of digestion model in vitro. The extraction rate was 74.9% and the residue rate was 27.8%. (3) The relative standard deviations of the analytical results of Ca contents in those two fractions were 2.56%and 9.10%, respectively, in the reproducibility tests. It was showed that the digestion model in vitro did not affect the reproduction quality significantly. Moreover, the deviation between the theoretical value and the summation value of the ex-traction and the residue rates was only 2.7%, which showed good availability of the digestion model in vitro to be used as a pre-treatment method in mineral element analysis process. (4) Relative standard deviation and recovery yield in the determination procedure were 0. 11 %(n=9)and 99. 2%, respectively.