中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2009年
1期
6-9,封4
,共5页
汪雨娜%牛忠英%司少艳%施生根%冯凯%史亮%党平%王睿%路浩军%吴国栋
汪雨娜%牛忠英%司少豔%施生根%馮凱%史亮%黨平%王睿%路浩軍%吳國棟
왕우나%우충영%사소염%시생근%풍개%사량%당평%왕예%로호군%오국동
失重模拟%成纤维细胞生长因子2%细胞增殖%牙周膜
失重模擬%成纖維細胞生長因子2%細胞增殖%牙週膜
실중모의%성섬유세포생장인자2%세포증식%아주막
Weightlessness simulation%Fibroblast growth factor 2%Cell proliferation%Periodontal ligament
目的 探讨在模拟失重环境下碱性成纤维细胞生长因子(bFGF)对人牙周膜成纤维细胞(hPDLF)增殖的影响. 方法 试验分对照组、模拟失重组和模拟失重加hPDLF组3组.采用酶消化组织块培养法分离、培养原代hPDLF.应用噻唑蓝比色法,检测模拟失重条件下,不同时间和不同浓度的bFGF对hPDLF增殖的影响. 结果 在模拟失重12h、24h组hPDLF增殖较对照组没有显著差别,在48h、72h模拟失重组较对照组显著降低,差异有统计学意义(t=7.303、8.668,P<0.01).模拟失重48 h时bFGF浓度在50～100μg/L范围内加bFGF组hPDLF增殖高于模拟失重组,差异有统计学意义(P<0.05),而bFGF浓度为1～10μg/L时,两组没有显著差别. 结论 模拟失重在48 h、72 h对hPDLF的增殖有抑制作用,模拟失重环境bFGF浓度在50～100μg/L范围内具有促进hPDLF增殖的效应.
目的 探討在模擬失重環境下堿性成纖維細胞生長因子(bFGF)對人牙週膜成纖維細胞(hPDLF)增殖的影響. 方法 試驗分對照組、模擬失重組和模擬失重加hPDLF組3組.採用酶消化組織塊培養法分離、培養原代hPDLF.應用噻唑藍比色法,檢測模擬失重條件下,不同時間和不同濃度的bFGF對hPDLF增殖的影響. 結果 在模擬失重12h、24h組hPDLF增殖較對照組沒有顯著差彆,在48h、72h模擬失重組較對照組顯著降低,差異有統計學意義(t=7.303、8.668,P<0.01).模擬失重48 h時bFGF濃度在50～100μg/L範圍內加bFGF組hPDLF增殖高于模擬失重組,差異有統計學意義(P<0.05),而bFGF濃度為1～10μg/L時,兩組沒有顯著差彆. 結論 模擬失重在48 h、72 h對hPDLF的增殖有抑製作用,模擬失重環境bFGF濃度在50～100μg/L範圍內具有促進hPDLF增殖的效應.
목적 탐토재모의실중배경하감성성섬유세포생장인자(bFGF)대인아주막성섬유세포(hPDLF)증식적영향. 방법 시험분대조조、모의실중조화모의실중가hPDLF조3조.채용매소화조직괴배양법분리、배양원대hPDLF.응용새서람비색법,검측모의실중조건하,불동시간화불동농도적bFGF대hPDLF증식적영향. 결과 재모의실중12h、24h조hPDLF증식교대조조몰유현저차별,재48h、72h모의실중조교대조조현저강저,차이유통계학의의(t=7.303、8.668,P<0.01).모의실중48 h시bFGF농도재50～100μg/L범위내가bFGF조hPDLF증식고우모의실중조,차이유통계학의의(P<0.05),이bFGF농도위1～10μg/L시,량조몰유현저차별. 결론 모의실중재48 h、72 h대hPDLF적증식유억제작용,모의실중배경bFGF농도재50～100μg/L범위내구유촉진hPDLF증식적효응.
Objective To evaluate the effects of basic fibroblast growth factor (bFGF) on the proliferation of human periodontal ligament fibroblast cells (hPDLF) under simulated microgravity.Methods Subjects were divided into control, simulated microgravity and simulated microgravity with bFGF groups. The primary cells were separated from human periodontal ligament by explants with enzymatic digestion. Methyl thiazolyl tetrazolium colorimetric assay was used to detect the effects of bFGF with various duration and concentration on proliferation activity of hPDLF under simulated microgravity. Results The proliferation activities of hPDLFs under simulated microgravity for 12 h and 24 h were not significantly different from that of the control, but significant for 48 h and 72 h exposure (t=7. 303, 8. 668, P<0.01). After 48 h exposure the proliferation activity of hPDLFs that with 50-100 μg/L bFGF in simulated microgravity with bFGF group was significantly increased comparing with that of simulated microgravity group (P < 0. 05). But there was no significant difference between two groups when 1-10 μg/L bFGF reached. Conclusions Simulated microgravity may inhibit the proliferation activity of hPDLF after 48 h and 72 h exposure. The concentration of bFGF within 50-100 μg/L may enhance the proliferative responses of hPDLF under simulated microgravity.
