CAJ | 학술논문

目的检测黑色素瘤抗原MAGE-4和MAGE-10 mRNA在中国人肝细胞癌（HCC）中的表达，为用这两种基因编码蛋白作为疫苗对HCC患者进行免疫治疗提供依据。方法用RT-PCR方法对48例HCC患者癌组织和相应癌旁肝组织、10例肝硬化组织和10例正常肝组织的MAGE-4和MAGE-10 mRNA表达情况进行研究，并对RT-PCR扩增产物中目的基因片段进行cDNA序列测定。结果 48例HCC患者中，有15例(31.3%)表达MAGE-4，14例(29.2%)表达MAGE-10 mRNA；而相应癌旁肝组织、肝硬化和正常肝组织均不表达。cDNA测序进一步证明，PCR产物中的目的基因片段为MAGE-4和MAGE-10 cDNA序列，两种MAGE基因的表达与肿瘤大小、甲胎蛋白(α-FP)水平等临床指标无相关关系。结论 MAGE-4和MAGE-10在HCC组织中呈特异性的表达，而在癌旁非瘤性肝组织中均不表达，这使得以此抗原用于HCC患者的免疫治疗成为可能。
목적검측흑색소류항원MAGE-4화MAGE-10 mRNA재중국인간세포암（HCC）중적표체，위용저량충기인편마단백작위역묘대HCC환자진행면역치료제공의거。방법용RT-PCR방법대48례HCC환자암조직화상응암방간조직、10례간경화조직화10례정상간조직적MAGE-4화MAGE-10 mRNA표체정황진행연구，병대RT-PCR확증산물중목적기인편단진행cDNA서렬측정。결과 48례HCC환자중，유15례(31.3%)표체MAGE-4，14례(29.2%)표체MAGE-10 mRNA；이상응암방간조직、간경화화정상간조직균불표체。cDNA측서진일보증명，PCR산물중적목적기인편단위MAGE-4화MAGE-10 cDNA서렬，량충MAGE기인적표체여종류대소、갑태단백(α-FP)수평등림상지표무상관관계。결론 MAGE-4화MAGE-10재HCC조직중정특이성적표체，이재암방비류성간조직중균불표체，저사득이차항원용우HCC환자적면역치료성위가능。
Objective　To investigate the expression of melanoma antigen MAGE-4 and MAGE-10 mRNA in Chinese human hepatocellular carcinoma (HCC).Methods　The expression of MAGE-4 and MAGE-10 mRNA in HCC tissues and the adjacent non-HCC liver tissues was studied using RT-PCR in 48 samples of HCC. Ten samples of cirrhosis and 10 samples of normal liver tissues were examined. The PCR products were sequenced.Results　Of the 48 HCC samples studied, 15 (31.3%) and 14 (29.2%) expressed MAGE-4 and MAGE-10 mRNA respectively. In contrast, none of the HCC adjacent non-tumorous liver tissues were MAGE-4 and MAGE-10 mRNA detectable. Nor did liver tissues from cirrhosis and normal liver samples. The expression of the two genes in HCC showed no correlation with the serum level of AFP and the tumor size.Conclusion　MAGE-4 and MAGE-10 mRNA are specifically expressed in Chinese HCC samples.