中草药
中草藥
중초약
CHINESE TRADITIONAL AND HERBAL DRUGS
2003年
5期
430-433
,共4页
红花%离体血管肌条%内皮细胞%钙内流
紅花%離體血管肌條%內皮細胞%鈣內流
홍화%리체혈관기조%내피세포%개내류
the dried flower of Carthamus tinctorius L.%isolated aortic strip%endothelium cells%calcium influx
目的观察红花Carthamus tinctorius(DFCT)水煎剂对血管肌条的舒张作用及机制.方法将家兔离体主动脉肌条放置于灌流肌槽中,记录其等长收缩.结果 DFCT对血管肌条静息张力无明显影响,但20mg/mLDFCT水煎剂与10-5mol/L乙酰胆碱相似,可使10-6mol/L去甲肾上腺素预收缩血管肌条产生明显的舒张作用.去除内皮细胞、10-4mol/L L-NNA或10-5mol/L甲烯蓝可减弱DFCT的舒张血管作用,但前列腺素合成抑制剂和β肾上腺素能受体阻断无明显影响.另外,40mg/mLDFCT水煎剂可明显抑制去内皮血管肌条去甲肾上腺和KCl的量效收缩反应,使其PD2值分别由对照组6.06±0.09和1.71±0.33变为5.07±0.08和1.35±0.20.结论 DFCT水煎剂可通过受体操纵Ca2+通道和电压依赖性Ca2+通道抑制外Ca2+内流,使血管肌条舒张,其作用与内皮释放的NO有关.
目的觀察紅花Carthamus tinctorius(DFCT)水煎劑對血管肌條的舒張作用及機製.方法將傢兔離體主動脈肌條放置于灌流肌槽中,記錄其等長收縮.結果 DFCT對血管肌條靜息張力無明顯影響,但20mg/mLDFCT水煎劑與10-5mol/L乙酰膽堿相似,可使10-6mol/L去甲腎上腺素預收縮血管肌條產生明顯的舒張作用.去除內皮細胞、10-4mol/L L-NNA或10-5mol/L甲烯藍可減弱DFCT的舒張血管作用,但前列腺素閤成抑製劑和β腎上腺素能受體阻斷無明顯影響.另外,40mg/mLDFCT水煎劑可明顯抑製去內皮血管肌條去甲腎上腺和KCl的量效收縮反應,使其PD2值分彆由對照組6.06±0.09和1.71±0.33變為5.07±0.08和1.35±0.20.結論 DFCT水煎劑可通過受體操縱Ca2+通道和電壓依賴性Ca2+通道抑製外Ca2+內流,使血管肌條舒張,其作用與內皮釋放的NO有關.
목적관찰홍화Carthamus tinctorius(DFCT)수전제대혈관기조적서장작용급궤제.방법장가토리체주동맥기조방치우관류기조중,기록기등장수축.결과 DFCT대혈관기조정식장력무명현영향,단20mg/mLDFCT수전제여10-5mol/L을선담감상사,가사10-6mol/L거갑신상선소예수축혈관기조산생명현적서장작용.거제내피세포、10-4mol/L L-NNA혹10-5mol/L갑희람가감약DFCT적서장혈관작용,단전렬선소합성억제제화β신상선소능수체조단무명현영향.령외,40mg/mLDFCT수전제가명현억제거내피혈관기조거갑신상선화KCl적량효수축반응,사기PD2치분별유대조조6.06±0.09화1.71±0.33변위5.07±0.08화1.35±0.20.결론 DFCT수전제가통과수체조종Ca2+통도화전압의뢰성Ca2+통도억제외Ca2+내류,사혈관기조서장,기작용여내피석방적NO유관.
Object To observe the vasodilatory effect of a water decoction of the dried flower of Carthamus tinctorius L. (DFCT) on rabbit thoracic aorta and its mechanism. Methods Strips of rabbit aortic smooth muscle were suspended in organ baths containing Kreb's solution, and then isometric tension was measured. Results DFCT did not change the resting tension of rabbit aortic strips. But similar to Ach (10-5 mol/L), DFCT (20 mg/mL) could cause an obvious relaxation in 10-6mol/L NA-precontracted arterial strips. The relaxant effect of DFCT was significantly reduced by removal of endothelium and 10-4mol/L L-NNA and 10-5mol/L methylene blue but not by prostaglandin synthase inhibitor and blockage of adrenergic β receptor. In addition, DFCT (40 mg/mL) inhibited NA and KCl cumulative concentration-response curves of aortic strips without endothelium, and changed the PD2 values for NA from (6.06±0.09) in control group to (5.07±0.08) and for KCl from (1.71±0. 33) in control group to (1.35±0.20),respectively. Conclusion These results suggest that the vasodilatory effect of DFCT in rabbit thoracic aorta may be related to the nitric oxide release from endothelium, and also probably due to inhibition of Ca2+ influx through receptor-operated and voltage-dependent calcium channels.
