安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
35期
17360-17361
,共2页
球根海棠%培养基%筛选
毬根海棠%培養基%篩選
구근해당%배양기%사선
Begonia×tuberhybrida Voss%Medium%Selection
[目的]筛选球根海棠叶培养中不同阶段的培养基配方.[方法]通过组织培养试验,从接种、继代增殖和生根培养3个阶段对球根海棠叶离体培养中的培养基配方进行筛选.[结果]在MS+6-BA 1.00 mg/L+NAA 0.20 mg/L+Ade 5.00 mg/L 的培养基上接种诱导分化丛生芽,诱导成芽率达100%,在诱导分化培养基中添加6-BA有利于芽的形成,在配方中添加Ade能促使诱导;在MS+6-BA 0.10 mg/L+NAA 0.01 mg/L的培养基上进行增殖,增殖系数高达40,且丛生芽整齐、健壮,最适用于生产;在1/2 MS+ IAA 0.80 mg/L培养基上生根壮苗,生根时间短、根系位置适宜、根系条数偏多,最为理想.[结论]筛选出了球根海棠叶离体培养时诱导阶段、增殖阶段和生根阶段的最佳培养基配方:在MS+6-BA 1.00 mg/L+NAA 0.20 mg/L+Ade 5.00 mg/L 的培养基上接种诱导分化丛生芽,然后在MS+6-BA 0.10 mg/L+NAA 0.01 mg/L的培养基上快速增殖,在1/2 MS+ IAA 0.80 mg/L培养基上生根壮苗,再出瓶培养成生产用苗.
[目的]篩選毬根海棠葉培養中不同階段的培養基配方.[方法]通過組織培養試驗,從接種、繼代增殖和生根培養3箇階段對毬根海棠葉離體培養中的培養基配方進行篩選.[結果]在MS+6-BA 1.00 mg/L+NAA 0.20 mg/L+Ade 5.00 mg/L 的培養基上接種誘導分化叢生芽,誘導成芽率達100%,在誘導分化培養基中添加6-BA有利于芽的形成,在配方中添加Ade能促使誘導;在MS+6-BA 0.10 mg/L+NAA 0.01 mg/L的培養基上進行增殖,增殖繫數高達40,且叢生芽整齊、健壯,最適用于生產;在1/2 MS+ IAA 0.80 mg/L培養基上生根壯苗,生根時間短、根繫位置適宜、根繫條數偏多,最為理想.[結論]篩選齣瞭毬根海棠葉離體培養時誘導階段、增殖階段和生根階段的最佳培養基配方:在MS+6-BA 1.00 mg/L+NAA 0.20 mg/L+Ade 5.00 mg/L 的培養基上接種誘導分化叢生芽,然後在MS+6-BA 0.10 mg/L+NAA 0.01 mg/L的培養基上快速增殖,在1/2 MS+ IAA 0.80 mg/L培養基上生根壯苗,再齣瓶培養成生產用苗.
[목적]사선구근해당협배양중불동계단적배양기배방.[방법]통과조직배양시험,종접충、계대증식화생근배양3개계단대구근해당협리체배양중적배양기배방진행사선.[결과]재MS+6-BA 1.00 mg/L+NAA 0.20 mg/L+Ade 5.00 mg/L 적배양기상접충유도분화총생아,유도성아솔체100%,재유도분화배양기중첨가6-BA유리우아적형성,재배방중첨가Ade능촉사유도;재MS+6-BA 0.10 mg/L+NAA 0.01 mg/L적배양기상진행증식,증식계수고체40,차총생아정제、건장,최괄용우생산;재1/2 MS+ IAA 0.80 mg/L배양기상생근장묘,생근시간단、근계위치괄의、근계조수편다,최위이상.[결론]사선출료구근해당협리체배양시유도계단、증식계단화생근계단적최가배양기배방:재MS+6-BA 1.00 mg/L+NAA 0.20 mg/L+Ade 5.00 mg/L 적배양기상접충유도분화총생아,연후재MS+6-BA 0.10 mg/L+NAA 0.01 mg/L적배양기상쾌속증식,재1/2 MS+ IAA 0.80 mg/L배양기상생근장묘,재출병배양성생산용묘.
[Objective] The selection of the optimum media for Begonia×tuberhybrida Voss leaf culture in vitro in different periods was experimented. [Method] The induction, subculture and rooting medium for its culture were experimented, respectively. [Results] In medium: MS + 6-BA 1.00 mg/L + NAA 0.20 mg/L + Ade 5.00 mg/L, the induction rate of bud Begonia×tuberhybrida leaf culture was 100%; 6-BA added in the medium was beneficial to bud formation and Ade added in the medium promoted the induction. The multiplication coefficient of bud was as high as 40% in the subculture medium: MS+6-BA 0.10 mg/L+NAA 0.01 mg/L and the bud growth was order and strong, which was suitable for production practice. In rooting medium: 1/2 MS+ IAA 0.80 mg/L, the root regenerated was strong and multiple, and the time of rooting was short. [Conclusion] The procedure of Begonia×tuberhybrida Voss leaf culture in vitro was as follows: the bud was induced in the medium: MS + 6-BA 1.00 mg/L + NAA 0.20 mg/L + Ade 5.00 mg/L; propagated in the medium: MS + 6-BA 0.10 mg/L+NAA 0.01 mg/L and rooted in the medium: 1/2 MS + IAA 0.80 mg/L.
