CAJ | 학술논문

以萝卜胞质不育型青花菜的幼嫩花序轴为外植体进行组织培养,可成功诱导出苗.将幼嫩花序轴接种到不同激素配比的诱导培养基上,均有愈伤组织产生,其中在MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L培养基培养效果最好,诱导率高达100%;用MS + NAA 0.01 mg/L +KT 0.2 mg/L + 6-BA 0.5 mg/L分化不定芽时,不定芽分化效果最好,芽分化率可达6.16;用1/2 MS + NAA 0.2 mg/L进行生根培养,生根率高达86.7%,平均根数13.5条,在一定的温度和湿度条件下培养,移栽成活率可达85%以上.
이라복포질불육형청화채적유눈화서축위외식체진행조직배양,가성공유도출묘.장유눈화서축접충도불동격소배비적유도배양기상,균유유상조직산생,기중재MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L배양기배양효과최호,유도솔고체100%;용MS + NAA 0.01 mg/L +KT 0.2 mg/L + 6-BA 0.5 mg/L분화불정아시,불정아분화효과최호,아분화솔가체6.16;용1/2 MS + NAA 0.2 mg/L진행생근배양,생근솔고체86.7%,평균근수13.5조,재일정적온도화습도조건하배양,이재성활솔가체85%이상.
Tissue culture of OguCMS Brassica oleracea L. var. varitalica Planch, which was done with its young rachides as explants, succeeded in seedling. After inoculating the young rachides against the induction medium compounded with different hormones, and it had the uppermost rachis inducing rate of 100% on the induction medium (MS + 6-BA 1.0mg/L + NAA 0.1mg/L). Seedlings showed favorable growth after being cultivated consequently on the enrichment medium (MS + 6-BA 1.0mg/L + NAA 0.01mg/L ) with a proliferation coefficient of 6.16. The rhizogenic rate was 86.7% with 13.5 pieces of roots in average on the rooting medium (1/2MS + NAA 0.2mg/L). Seedlings survival rate was up to 85% in certain temperature and humidity condition.