CAJ | 학술논문

[目的]测定石参中总黄酮含量并对其抗氧化性进行研究.[方法]采用超声波提取技术用体积分数为80%的乙醇提取石参中的总黄酮,分光光度法测定石参中的总黄酮类含量,研究石参中黄酮类化合物对超氧自由基、羟基自由基的清除作用.[结果]石参中总黄酮含量为0.742 5%,石参中黄酮类化合物对自由基清除作用随着黄酮浓度的增大而表现出明显的量效关系.总黄酮浓度为9.791×10~(-4) mg/ml时对超氧自由基的清除率最高,为48.91%;浓度为0.009 9 mg/ml时对羟基自由基的清除率最高,达到83.76%.[结论]石参总黄酮对超氧自由基、羟基自由基均具有一定的清除作用.
[목적]측정석삼중총황동함량병대기항양화성진행연구.[방법]채용초성파제취기술용체적분수위80%적을순제취석삼중적총황동,분광광도법측정석삼중적총황동류함량,연구석삼중황동류화합물대초양자유기、간기자유기적청제작용.[결과]석삼중총황동함량위0.742 5%,석삼중황동류화합물대자유기청제작용수착황동농도적증대이표현출명현적량효관계.총황동농도위9.791×10~(-4) mg/ml시대초양자유기적청제솔최고,위48.91%;농도위0.009 9 mg/ml시대간기자유기적청제솔최고,체도83.76%.[결론]석삼총황동대초양자유기、간기자유기균구유일정적청제작용.
[Objective] The aim was to determine total flavonoids in Eremurus chinens and study its antioxidant. [Method] The total flavonoids in E. chinens were extracted by volume fraction of 80% ethanol and using ultrasonic extraction technology. The content was determined by spectrophotometric method. And the removal effect of flavonoids on superoxide radical, hydroxyl radical was studied. [Result] The content of flavonoids in E. chinens was 0.742 5%. With the increasing of flavonoids, the removal effect on free radical became increasingly obvious. When concentration of total flavonoid is 9.791×10~(-4) mg/ml, the scavenging rate on superoxide radical was highest, up to 48.91%; when concentration is 0.009 9 mg/ml, the scavenging rate on hydroxyl radical was highest, up to 83.76%. [Conclusion] Total flavonoid in E. chinens has removal effect on superoxide radical and hydroxyl radical.