CAJ | 학술논문

[目的]对已知半胱氨酸蛋白酶抑制剂基因编码蛋白的分子量、等电点、信号肽、结构域等进行分析.[方法]在NCBI中检索半胱氨酸蛋白酶抑制剂基因,下载相应的氨基酸序列.采用SMART软件预测结构域,用SingalP程序查找信号肽,用TMHMM程序搜寻预测跨膜区.多序列比对采用CLUSTAL W程序.运用MEGA3.1软件,采用Neighbor-joining 法构建进化树.[结果]半胱氨酸蛋白酶抑制A(Homo sapiens)、半胱氨酸蛋白酶抑制M(H. sapiens)、半胱氨酸蛋白酶抑制F(H. sapiens)、半胱氨酸蛋白酶抑制(Mus musculus)、半胱氨酸蛋白酶抑制C(M. musculus)、半胱氨酸蛋白酶抑制F(Rattus norvegicus)、半胱氨酸蛋白酶抑制C(R. norvegicus)、半胱氨酸蛋白酶抑制S(R. norvegicus)、半胱氨酸蛋白酶抑制I(Zea mays)、半胱氨酸蛋白酶抑制(Brugia malayi)、半胱氨酸蛋白酶抑制(Onchocerca volvulus)和半胱氨酸蛋白酶抑制(Acanthocheilonema viteae)有信号肽,其余的半胱氨酸蛋白酶抑制基因没有信号肽,TMHMM程序搜寻结果显示,这些半胱氨酸蛋白酶抑制都没有跨膜区,均为胞外蛋白.SMART软件分析结果表明它们均含有1个高度保守的半胱氨酸蛋白酶抑制剂结构域.多序列比对结果表明半胱氨酸蛋白酶抑制剂基因存在高度保守的QxVxG基序,意味着该基序可能对半胱氨酸蛋白酶抑制剂的抑制活性具有重要意义.系统进化分析可预示半胱氨酸蛋白酶抑制半胱氨酸蛋白酶活性在进化过程中可能也是保守的.[结论]该研究可为半胱氨酸蛋白酶抑制剂抑制半胱氨酸蛋白酶的功能研究方面提供理论参考.
[목적]대이지반광안산단백매억제제기인편마단백적분자량、등전점、신호태、결구역등진행분석.[방법]재NCBI중검색반광안산단백매억제제기인,하재상응적안기산서렬.채용SMART연건예측결구역,용SingalP정서사조신호태,용TMHMM정서수심예측과막구.다서렬비대채용CLUSTAL W정서.운용MEGA3.1연건,채용Neighbor-joining 법구건진화수.[결과]반광안산단백매억제A(Homo sapiens)、반광안산단백매억제M(H. sapiens)、반광안산단백매억제F(H. sapiens)、반광안산단백매억제(Mus musculus)、반광안산단백매억제C(M. musculus)、반광안산단백매억제F(Rattus norvegicus)、반광안산단백매억제C(R. norvegicus)、반광안산단백매억제S(R. norvegicus)、반광안산단백매억제I(Zea mays)、반광안산단백매억제(Brugia malayi)、반광안산단백매억제(Onchocerca volvulus)화반광안산단백매억제(Acanthocheilonema viteae)유신호태,기여적반광안산단백매억제기인몰유신호태,TMHMM정서수심결과현시,저사반광안산단백매억제도몰유과막구,균위포외단백.SMART연건분석결과표명타문균함유1개고도보수적반광안산단백매억제제결구역.다서렬비대결과표명반광안산단백매억제제기인존재고도보수적QxVxG기서,의미착해기서가능대반광안산단백매억제제적억제활성구유중요의의.계통진화분석가예시반광안산단백매억제반광안산단백매활성재진화과정중가능야시보수적.[결론]해연구가위반광안산단백매억제제억제반광안산단백매적공능연구방면제공이론삼고.
[Objective] The molecular weight, isoelectric point, signal peptide, domain and other properties of the encoding protein of the known cystatin genes were analyzed. [Method] Cystatin genes were searched in NCBI and the related amino acids sequences were downloaded. SMART software was used to predict the domain. SingalP program was used to search signal peptide. TMHMM program was used to search and predict the transmembrane domain. CLUSTAL W program was used to make multiple sequence alignment. Using MEGA3.1 software, the phylogenetic tree was constructed by using Neighbor-joining method. [Result] Multiple sequence alignment showed that cystatin genes had QxVxG base sequences with high conservation, which indicated that the inhibition activity of cystatin had important significance. The phylogenetic analysis might predict that inhibitor activity for cysteine proteinase might be conserved in the evolution process. [Conclusion] The research provided theoretical basis for the research on the inhibition of cystatin on cysteine proteinase.