CAJ | 학술논문

目的分析骨髓增生异常综合征( MDS)及再生障碍性贫血(AA)患者外周血单个核细胞( PBMC)蛋白激酶Cθ (PKCθ)的表达及Th1/Th2、Tc1/Tc2的变化,探讨PKC θ在MDS及AA发病机制中的作用.方法收集外周血标本,MDS-RA 14例,AA 15例,健康人30名,分离PBMC,实时荧光定量PCR方法检测PKC θ mRNA表达水平；流式细胞术检测Th1/Th2类细胞因子和Tc1/Tc2类细胞因子的表达水平.结果 MDS及AA患者PBMC中PKC θ mRNA相对表达量较健康对照组升高(AA组为23.54±1.01,MDS组为23.76±1.58；健康对照组为27.12±1.12,P=0.004)；不同组间Th1、Tc1类细胞因子表达差异有统计学意义(均P＜ 0.05).不同组间Th2、Tc2类细胞因子白细胞介素(IL)-4、IL-6、IL-10分泌水平与健康对照组差异无统计学意义(均P＞0.05)；MDS及AA患者组间PKC θ mRNA表达及Th1/Th2,Tc1/Tc2类细胞因子表达差异无统计学意义(均P＞0.05).结论 MDS及AA患者PBMC中PKC θ表达增高,Th1、Tc1细胞相关细胞因子分泌增加.
목적 분석골수증생이상종합정( MDS)급재생장애성빈혈(AA)환자외주혈단개핵세포( PBMC)단백격매Cθ (PKCθ)적표체급Th1/Th2、Tc1/Tc2적변화,탐토PKC θ재MDS급AA발병궤제중적작용.방법 수집외주혈표본,MDS-RA 14례,AA 15례,건강인30명,분리PBMC,실시형광정량PCR방법검측PKC θ mRNA표체수평；류식세포술검측Th1/Th2류세포인자화Tc1/Tc2류세포인자적표체수평.결과 MDS급AA환자PBMC중PKC θ mRNA상대표체량교건강대조조승고(AA조위23.54±1.01,MDS조위23.76±1.58；건강대조조위27.12±1.12,P=0.004)；불동조간Th1、Tc1류세포인자표체차이유통계학의의(균P＜ 0.05).불동조간Th2、Tc2류세포인자백세포개소(IL)-4、IL-6、IL-10분비수평여건강대조조차이무통계학의의(균P＞0.05)；MDS급AA환자조간PKC θ mRNA표체급Th1/Th2,Tc1/Tc2류세포인자표체차이무통계학의의(균P＞0.05).결론 MDS급AA환자PBMC중PKC θ표체증고,Th1、Tc1세포상관세포인자분비증가.
Objective To analyse expression levels of protein kinase C θ (PKC θ ) and its association with Th1/Th2,Tc1/Tc2 cytokines in peripheral blood mononuclear cells (PBMC) of patients with myelodysplastic syndromes and aplastic anemia (AA),and understand the pathogenesis of MDS and AA.Methods Fourteen patients with MDS-RA, fifteen patients with AA, and thirty health controls of PBMC were collected.mRNA expression levels of PKC θ were measured by RTQ-PCR,and the expression levels of Th1/Th2,Tc1/Tc2 cytokines were measured by the flow cytometry. Results The expression PKC θ mRNA (AA group:23.54±1.01,MDS group:23.76±1.58 ;health control group:27.12±1.12, P=0.004) and Th1and Tc1cytokines were statistically significant of among groups (all P＜0.05). Th2 and Tc2 cytokines were no statistical difference among groups (allP＞0.05). There were no statistical difference in the PBMC of PKC θ mRNA and Th1/Th2,Tc1/Tc2 cell cytokine (allP＞0.05) between MDS and AA.Conclusions The expression levels of PKCθmRNA and Th1,Tc1cells correlated cytokine in MDS and AA patients of PBMC are increases.