中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2009年
18期
1-4
,共4页
谷杭芝%陈慧君%郑飞云%熊术道
穀杭芝%陳慧君%鄭飛雲%熊術道
곡항지%진혜군%정비운%웅술도
宫颈肿瘤%eIF-2激酶B
宮頸腫瘤%eIF-2激酶B
궁경종류%eIF-2격매B
Uterine cervical neoplasms%eIF-2 Kinase B
目的 检测磷脂酰肌醇3激酶(PI3K)、磷酸化蛋白激酶B(P-AKT)、蛋白激酶B(AKT)在宫颈癌中的表达,进一步探讨其与宫颈癌发生、发展的关系.方法 应用蛋白免疫印迹技术检测66例宫颈标本(包括33例宫颈癌、23例宫颈上皮内瘤样病变、10例正常宫颈组织)PI3K、AKT、P-AKT表达情况,并结合临床资料分析其临床意义.结果 宫颈癌、宫颈上皮内瘤样病变、正常宫颈组织中PI3K表达量分别为1.3880±0.0435、0.5330±0.0939、0.2427±0.0888,三者比较差异有统计学意义;P-AKT/AKT分别为5.8702±0.0543、5.0755±0.0888、3.8353±0.0056,三者比较差异无统计学意义.宫颈鳞癌与宫颈腺癌和腺鳞癌的P-AKT/AKT分别为6.7823±0.7745和0.7621±0.0709,宫颈鳞癌P-AKT/AKT表达明显高于宫颈腺癌和腺鳞癌,差异有统计学意义.结论 PI3K过表达与宫颈癌的发生有一定的关系;P-AKT/AKT高表达与宫颈癌的病理类型有关.
目的 檢測燐脂酰肌醇3激酶(PI3K)、燐痠化蛋白激酶B(P-AKT)、蛋白激酶B(AKT)在宮頸癌中的錶達,進一步探討其與宮頸癌髮生、髮展的關繫.方法 應用蛋白免疫印跡技術檢測66例宮頸標本(包括33例宮頸癌、23例宮頸上皮內瘤樣病變、10例正常宮頸組織)PI3K、AKT、P-AKT錶達情況,併結閤臨床資料分析其臨床意義.結果 宮頸癌、宮頸上皮內瘤樣病變、正常宮頸組織中PI3K錶達量分彆為1.3880±0.0435、0.5330±0.0939、0.2427±0.0888,三者比較差異有統計學意義;P-AKT/AKT分彆為5.8702±0.0543、5.0755±0.0888、3.8353±0.0056,三者比較差異無統計學意義.宮頸鱗癌與宮頸腺癌和腺鱗癌的P-AKT/AKT分彆為6.7823±0.7745和0.7621±0.0709,宮頸鱗癌P-AKT/AKT錶達明顯高于宮頸腺癌和腺鱗癌,差異有統計學意義.結論 PI3K過錶達與宮頸癌的髮生有一定的關繫;P-AKT/AKT高錶達與宮頸癌的病理類型有關.
목적 검측린지선기순3격매(PI3K)、린산화단백격매B(P-AKT)、단백격매B(AKT)재궁경암중적표체,진일보탐토기여궁경암발생、발전적관계.방법 응용단백면역인적기술검측66례궁경표본(포괄33례궁경암、23례궁경상피내류양병변、10례정상궁경조직)PI3K、AKT、P-AKT표체정황,병결합림상자료분석기림상의의.결과 궁경암、궁경상피내류양병변、정상궁경조직중PI3K표체량분별위1.3880±0.0435、0.5330±0.0939、0.2427±0.0888,삼자비교차이유통계학의의;P-AKT/AKT분별위5.8702±0.0543、5.0755±0.0888、3.8353±0.0056,삼자비교차이무통계학의의.궁경린암여궁경선암화선린암적P-AKT/AKT분별위6.7823±0.7745화0.7621±0.0709,궁경린암P-AKT/AKT표체명현고우궁경선암화선린암,차이유통계학의의.결론 PI3K과표체여궁경암적발생유일정적관계;P-AKT/AKT고표체여궁경암적병리류형유관.
Objective To determine the expression of phosphatidyhnositol 3-kinase (PI3K), phosphorylated protein kinase B (P-AKT) and protein kinase B (AKT) in cervical cancer and explore the correlations with the genesis and development of cervical cancer. Methods Western blot was used to detect the expression of PI3K, P-AKT and AKT proteins in 66 cases of cervical tissues, including 33 cervical cancers, 23 cervical intraepithelial cancer, 10 normal cervical tissues, and analyzed the clinical significances according to clinical informations. Results In cervical cancers, cervical intraepithelial cancer and normal cervical tissues, the expression of PI3K protein were 1.3880±0.0435, 0.5330±0.0939, 0.2427±0.0888 and had significant differences among them, but there was no significant difference in the ratio of P-AKT and AKT, which were 5.8702±0.0543, 5.0755±0.0888, 3.8353±0.0056. The ratios of P-AKT and AKT had association with histological type, the ratios in squamous cancer of the cervix and in adenocancer and denosquamous cancer were 6.7823±0.7745 and 0.7621±0.0709, and the ratios were much higher in squamous cancer of the cervix than adenocancer and denosquamous cancer. Conclusions Overexpression of PI3K protein is involved in the occurrence of human cervical cancer. The ratio of P-AKT and AKT plays a more important role in squamous cancer of the cervix.
