CAJ | 학술논문

目的探讨人类白细胞抗原G(HLA-G)基因第8外显子14 bp缺失多态性与重度子痫前期发病的关系.方法选择2008年10月至2009年2月在郑州大学第三附属医院妇产科住院的42例孕晚期重度子痫前期孕妇及其新生儿为重度子痫前期组.另选择同期正常孕晚期孕妇及其新生儿45例为健康晚孕组,两组孕妇均为汉族居民.采用PCR技术对两组孕妇及其新生儿进行HLA-G基因第8外显子14 bp缺失多态性的等位基因分析,分别比较两组孕妇及其新生儿之间等位基因及基因型的频率分布,通过母、儿基因型配伍,比较两组间基因型配伍频率分布的差异.结果 (1)重度子痫前期组中母、儿均为14 bp缺失纯合子(-14 bp/-14 bp)的基因型配伍的频率为14%(6/42),显著低于健康晚孕组的33%(15/45),两组比较,差异有统计学意义(P=0.038);(2)重度子痫前期组孕妇HIA-G第8外显子14 bp缺失多态性的等位基因频率、基因型频率与健康晚孕组比较,差异均尤统计学意义(P>0.05);(3)重度子痫前期组新生儿HLA-G第8外显子14 bp缺失多态性等位基因+14 bp频率为44%(37/84)、-14 bp为56%(47/84),健康晚孕组新生儿+14 bp为30%(27/90)、-14 bp为70%(63/90),两组比较,差异虽无统计学意义,但存在差异性趋势(P=0.055);重度子痫前期组新生儿(-14 bp/-14 bp)基因型频率为29%(12/42),与健康晚孕组的49%(22/45)相比,存在差异性趋势(P=0.052).结论中国汉族孕妇中,HIJA-G第8外显子14 bp缺失多态性与重度子痫前期的发病有关;母、儿均为缺失纯合子(-14 bp/-14 bp)基因型配伍者,发生重度子痫前期的风险会降低.
목적 탐토인류백세포항원G(HLA-G)기인제8외현자14 bp결실다태성여중도자간전기발병적관계.방법 선택2008년10월지2009년2월재정주대학제삼부속의원부산과주원적42례잉만기중도자간전기잉부급기신생인위중도자간전기조.령선택동기정상잉만기잉부급기신생인45례위건강만잉조,량조잉부균위한족거민.채용PCR기술대량조잉부급기신생인진행HLA-G기인제8외현자14 bp결실다태성적등위기인분석,분별비교량조잉부급기신생인지간등위기인급기인형적빈솔분포,통과모、인기인형배오,비교량조간기인형배오빈솔분포적차이.결과 (1)중도자간전기조중모、인균위14 bp결실순합자(-14 bp/-14 bp)적기인형배오적빈솔위14%(6/42),현저저우건강만잉조적33%(15/45),량조비교,차이유통계학의의(P=0.038);(2)중도자간전기조잉부HIA-G제8외현자14 bp결실다태성적등위기인빈솔、기인형빈솔여건강만잉조비교,차이균우통계학의의(P>0.05);(3)중도자간전기조신생인HLA-G제8외현자14 bp결실다태성등위기인+14 bp빈솔위44%(37/84)、-14 bp위56%(47/84),건강만잉조신생인+14 bp위30%(27/90)、-14 bp위70%(63/90),량조비교,차이수무통계학의의,단존재차이성추세(P=0.055);중도자간전기조신생인(-14 bp/-14 bp)기인형빈솔위29%(12/42),여건강만잉조적49%(22/45)상비,존재차이성추세(P=0.052).결론 중국한족잉부중,HIJA-G제8외현자14 bp결실다태성여중도자간전기적발병유관;모、인균위결실순합자(-14 bp/-14 bp)기인형배오자,발생중도자간전기적풍험회강저.
Objective To investigate the relationship between human leukocyte antigen-G( HLA-G) gene Exon 8 14 bp deletion polymorphism and the pathogenesis of severe pre-eclampsia.Methods Forty-two pregnant women with severe pre-eclampsia,who admitted to the Third Affiliated Hospital of Zhengzhou University from October 2008 to February 2009,and their newborns were chosen as the severe pre-eclampsia group.Another 45 healthy gravidas at the third trimester and their newborns were chosen as the control.All gravidas in both groups were Han Nationality.HLA-G Exon 8 genotyping was detected by PCR in both groups and the allele frequencies and genotype frequencies were compared between the two groups.The genotype frequencies of maternal-neonatal pairs were also analyzed.Results ( 1 ) In the severe pre-eclampsia group,14% of the maternal-neonatal pairs were homozygote of 14 bp deletion,and significantly higher frequency 33% (15/45) was found in the control group (P =0.038).(2) No significant difference was found in the allele frequencies and genotype frequencies of HLA-G 14 bp deletion polymorphism among all the mothers between the two groups (P >0.05).(3) The + 14 bp and-14 bp allele frequencies of HLA-G 14 bp deletion polymorphism in newborns in the severe pre-eclampsia group were 44% (37/84) and56% (47/84),respectively,and 30% (27/90) and 70% (63/90) in the control group.Although there was no significant difference between the two groups,but differences in trends was identified (χ2= 3.678 P = 0.055) ; The genotype (-14 bp/-14 bp) frequency of neonates in the severe pre-eclampsia group showed no difference compared with that in the control group[29% (12/42) vs 49% (22/45)],but differences in trends was also found (P =0.052).Conclusions HLA-G 14 bp deletion polymorphism is associated with the susceptibility of severe pre-eclampsia in Chinese Han nationality.Maternal-fetal genotype pairs of-14 bp/-14 bp may have reduced risk of severe pre-eclampsia.