中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
12期
921-924
,共4页
李旭东%陈建雄%刘移民%苏世标%郭晓
李旭東%陳建雄%劉移民%囌世標%郭曉
리욱동%진건웅%류이민%소세표%곽효
噪声,职业性%超氧化物歧化酶%多态性,单核苷酸%疾病遗传易感性
譟聲,職業性%超氧化物歧化酶%多態性,單覈苷痠%疾病遺傳易感性
조성,직업성%초양화물기화매%다태성,단핵감산%질병유전역감성
Noise,occupation%Superoxide dismutase%Polymorphism,single nucleotide%Genetic predisposition to disease
目的 探讨中国汉族人群中噪声性听力损失(NIHL)易感性与超氧化物歧化酶基因(SODI) rs1041740、rs2070424、rs10432782和rs4998557单核苷酸多态性(SNP)之间的关联.方法 利用病例-对照研究,通过比较同一噪声暴露强度作业人员的左耳3000 Hz频段听阈位移情况,筛选出听阈位移最大的10％个体作为该研究的易感人群组,共201例;听阈位移最小的10％个体作为耐受人群组,共202例,并进行相关的职业卫生调查和问卷调查.抽取易感人群和耐受人群空腹外周静脉血5 ml用于常规方法抽提基因组DNA,TaqMan探针法化学荧光等位基因鉴别试验检测SNP.结果 SODI基因rs2070424 A等位基因是NIHL的保护因素,与G等位基因相比,OR值为0.67,95％CI为0.50～0.88,携带AA基因型的个体患NIHL的风险是GG基因型的0.48倍,95％CI为0.26～0.79.校正混杂因素后,OR值为0.44,95％CI为0.25～0.78.结论 在中国汉族人群中,NIHL易感性与SODI rs2070424位点SNP有关.
目的 探討中國漢族人群中譟聲性聽力損失(NIHL)易感性與超氧化物歧化酶基因(SODI) rs1041740、rs2070424、rs10432782和rs4998557單覈苷痠多態性(SNP)之間的關聯.方法 利用病例-對照研究,通過比較同一譟聲暴露彊度作業人員的左耳3000 Hz頻段聽閾位移情況,篩選齣聽閾位移最大的10％箇體作為該研究的易感人群組,共201例;聽閾位移最小的10％箇體作為耐受人群組,共202例,併進行相關的職業衛生調查和問捲調查.抽取易感人群和耐受人群空腹外週靜脈血5 ml用于常規方法抽提基因組DNA,TaqMan探針法化學熒光等位基因鑒彆試驗檢測SNP.結果 SODI基因rs2070424 A等位基因是NIHL的保護因素,與G等位基因相比,OR值為0.67,95％CI為0.50～0.88,攜帶AA基因型的箇體患NIHL的風險是GG基因型的0.48倍,95％CI為0.26～0.79.校正混雜因素後,OR值為0.44,95％CI為0.25～0.78.結論 在中國漢族人群中,NIHL易感性與SODI rs2070424位點SNP有關.
목적 탐토중국한족인군중조성성은력손실(NIHL)역감성여초양화물기화매기인(SODI) rs1041740、rs2070424、rs10432782화rs4998557단핵감산다태성(SNP)지간적관련.방법 이용병례-대조연구,통과비교동일조성폭로강도작업인원적좌이3000 Hz빈단은역위이정황,사선출은역위이최대적10％개체작위해연구적역감인군조,공201례;은역위이최소적10％개체작위내수인군조,공202례,병진행상관적직업위생조사화문권조사.추취역감인군화내수인군공복외주정맥혈5 ml용우상규방법추제기인조DNA,TaqMan탐침법화학형광등위기인감별시험검측SNP.결과 SODI기인rs2070424 A등위기인시NIHL적보호인소,여G등위기인상비,OR치위0.67,95％CI위0.50～0.88,휴대AA기인형적개체환NIHL적풍험시GG기인형적0.48배,95％CI위0.26～0.79.교정혼잡인소후,OR치위0.44,95％CI위0.25～0.78.결론 재중국한족인군중,NIHL역감성여SODI rs2070424위점SNP유관.
Objective To observe the association between SNPs in SODI (rs1041740,rs2070424,rs10432782 and rs4998557) and noise-induced hearing loss in Chinese Han population.Methods A casecontrol study was used to study the effects of environmental risk factors on the susceptibility to noise induced hearing loss (NIHL) in 201sensitive workers and 202 resistant workers.A questionnaire was designed to carry out an investigation,and an occupational health survey was used to identify the occupational risk factors.Genomic DNA was extracted from peripheral blood cells using standard procedures of Takara kit,and 5 ml blood was from each subject.SNPs were detected using standard procedures of TaqMan probe allele identification method.Results In SODI gene,the A allele of rs2070424 was a protective factor of NIHL,compared with the G allele (OR=0.67,95％CI:0.50～ 0.88).The risk with NIHL in suhjects with AA genotype was significantly lower than that in subjects with GG genotype(OR=0.48,95％CI:0.26～0.79).After adjusting the confusion factors,OR was 0.44 and 95％CIwas 0.25～0.78.Conclusion In Chinese Han population,the SNP of rs2070424 in SODL may be associated with the susceptibility to NIHL.
