CAJ | 학술논문

目的探讨N0期大肠癌淋巴结微转移的检测及其临床意义.方法采用荧光定量PCR方法检测62例行根治术的N0期大肠癌患者的548枚淋巴结中细胞角蛋白20(cytokeratin,CK20)mRNA的表达.结果本组62例N0期大肠癌患者的548枚淋巴结中,有24例(39%)共55枚(10.0%)淋巴结中检出微转移.其中第Ⅰ、第Ⅱ、第Ⅲ组淋巴结微转移率分别是15.8%、5.0%和3.3%.微转移与患者年龄、性别、肿瘤大小、肿瘤部位和分化程度等尤关,但与肿瘤浸润深度相关(x2=5.462,P<0.05).结论检测N0期大肠癌淋巴结中CK20mRNA的表达可对精确临床分期、了解淋巴结微转移分布及制定合理的治疗方案提供一定的理论依据.
목적 탐토N0기대장암림파결미전이적검측급기림상의의.방법 채용형광정량PCR방법 검측62례행근치술적N0기대장암환자적548매림파결중세포각단백20(cytokeratin,CK20)mRNA적표체.결과 본조62례N0기대장암환자적548매림파결중,유24례(39%)공55매(10.0%)림파결중검출미전이.기중제Ⅰ、제Ⅱ、제Ⅲ조림파결미전이솔분별시15.8%、5.0%화3.3%.미전이여환자년령、성별、종류대소、종류부위화분화정도등우관,단여종류침윤심도상관(x2=5.462,P<0.05).결론 검측N0기대장암림파결중CK20mRNA적표체가대정학림상분기、료해림파결미전이분포급제정합리적치료방안제공일정적이론의거.
Objective To study lymph node micrometastasis in N0 colorectal cancer patients and its clinical significance. Methods In this study, 548 lymph nodes obtained from 62 cases of No colorectal cancer undergoing curative operation were examined by fluorescent quantity polymerase chain reaction assay for the expression of cytokeratin 20 (CK20) mRNA. Results Micrometastasis was detected in 55 lymph nodes (10.0% ) of 24 cases (39%). According to lymph node anatomical locations, micrometastasis was identified in 15. 8%, 5.0% and 3.3% lymph nodes in group Ⅰ ,Ⅱ and Ⅲ (grouped by the distance from the tumor), respectively. Micrometastasis was correlated with invasion depth of primary tumor, but was notrelated to gender, age, tumor size, tumor site and differentiation. Conclusions The expression of CK20mRNA in lymph nodes in patients with No coloreetal cancer could be used to improve the accuracy of clinical staging, and provide information for rational adjuvant therapy.