CAJ | 학술논문

目的研究整合子在多重耐药阴沟肠杆菌中的分布和所起的作用.方法用Kirby-Bauer(K-B)药敏法分析40株多重耐药阴沟肠杆菌的耐药性,采用聚合酶链反应(PCR)技术检测耐药基因.结果 40株阴沟肠杆菌中有28(70.0%)株含有Ⅰ类整合子,它们对10种抗菌药物的耐药率依次为亚胺培南(7.5%)、美罗培南(5.0%)、哌拉西林/他唑巴坦(75.0%)、阿米卡星(55.0%)、头孢吡肟(12.5%)、头孢他啶(52.5%)、头孢噻肟(55.0%)、头孢曲松(90.0%)、替卡西林/克拉维酸(81.8%)、环丙沙星(85.0%).结论该院临床分离的阴沟肠杆菌多重耐药严重,Ⅰ类整合酶基因在多重耐药机制中起着重要的作用.
목적 연구정합자재다중내약음구장간균중적분포화소기적작용.방법 용Kirby-Bauer(K-B)약민법분석40주다중내약음구장간균적내약성,채용취합매련반응(PCR)기술검측내약기인.결과 40주음구장간균중유28(70.0%)주함유Ⅰ류정합자,타문대10충항균약물적내약솔의차위아알배남(7.5%)、미라배남(5.0%)、고랍서림/타서파탄(75.0%)、아미잡성(55.0%)、두포필우(12.5%)、두포타정(52.5%)、두포새우(55.0%)、두포곡송(90.0%)、체잡서림/극랍유산(81.8%)、배병사성(85.0%).결론 해원림상분리적음구장간균다중내약엄중,Ⅰ류정합매기인재다중내약궤제중기착중요적작용.
Objective To investigate the distribution and effect of class Ⅰ integron in 40 strains of clinical multi-resistant Enterobacter cloacae. Methods Kirby-Bauer method was used to analyze the drug resistance of 40 strains of multi-resistant Enterobacter cloacae and the drug resistance gene was analyzed by polymerase chain reaction (PCR). Results 28 strains (70.0%) of 40 clinical isolates were observed to contain class Ⅰ integron. Drug resistance test showed their resistance rate to 10 kind of an-tibaeterials as follow: 7.5% to imipenem, 5.0% to meropenem, 75.0% to piperacillin-tazobactam,55.0% to amikacin, 12.5% to cefepime, 52.5% to ceftazidime, 55.0% to cefotaxime, 90.0% to ceftriaxone;81.8% to ticarcillin-clavutanic acid, and 85.0% to ciprofloxacin. Conclusion This study shows that the resistance situation of Enterobacter cloacae isolates is very serious, and class Ⅰ integron may play an important role in the muhidrug resistant mechanism.