CAJ | 학술논문

背景:有学者认为手术增加了颈椎不稳定的风险,后纵韧带的张力有可能改变体内生长因子表达.然而两者是否存在关系还没有定论.骨形态发生蛋白(bone morphogenetic protein,BMP)4是具有独立异位成骨作用的细胞因子之一.目的:观察颈椎后路减压对颈椎后纵韧带中骨形态发生蛋白4 mRNA表达的影响.设计、时间及地点:随机对照动物实验,于2005-01/2006-12在泰山医学院完成.材料:清洁级成年SD大鼠48只,雌雄不拘,体质量270～350 g.方法:将大鼠随机分为3组.手术组:行C3～6全椎板切除减压加椎旁肌切断手术;假手术组:仅作皮肤切口;空白组:不作任何处理为.于处理后1,2,4,8周分批处死动物,取其后纵韧带.主要观察指标:采用半定量反转录-聚合酶链反应方法检测组织骨形态发生蛋白4 mRNA表达水平.结果:空白组及假手术组未见明显骨形态发生蛋白4 mRNA的表达;手术组于手术后1周表达明显,持续到8周.结论:颈椎后路减压手术可以导致后纵韧带早期细胞骨形态发生蛋白4 mRNA表达上升,内源性的骨形态发生蛋白4可能导致手术后韧带骨化发展.
배경:유학자인위수술증가료경추불은정적풍험,후종인대적장력유가능개변체내생장인자표체.연이량자시부존재관계환몰유정론.골형태발생단백(bone morphogenetic protein,BMP)4시구유독립이위성골작용적세포인자지일.목적:관찰경추후로감압대경추후종인대중골형태발생단백4 mRNA표체적영향.설계、시간급지점:수궤대조동물실험,우2005-01/2006-12재태산의학원완성.재료:청길급성년SD대서48지,자웅불구,체질량270～350 g.방법:장대서수궤분위3조.수술조:행C3～6전추판절제감압가추방기절단수술;가수술조:부작피부절구;공백조:불작임하처리위.우처리후1,2,4,8주분비처사동물,취기후종인대.주요관찰지표:채용반정량반전록-취합매련반응방법검측조직골형태발생단백4 mRNA표체수평.결과:공백조급가수술조미견명현골형태발생단백4 mRNA적표체;수술조우수술후1주표체명현,지속도8주.결론:경추후로감압수술가이도치후종인대조기세포골형태발생단백4 mRNA표체상승,내원성적골형태발생단백4가능도치수술후인대골화발전.
BACKGROUND: It has been thought that posterior cervical decompression increases the risk of cervical instability and the stress on posterior longitudinal ligament may change growth factor expression in vivo. However, the association between BMP-4 expression and heterotopic bone formation has not been confirmed. Bone morphogenetic protein 4 (BMP-4) is one of factors that can lead to heterotopic bone formation alone.OBJECTIVE: To observe the effects of posterior cervical decompression on BMP-4 mRNA expression in the posterior longitudinal ligament.DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed in Taishan Medical College between January 2005 and December 2006.MATERIALS: A total of 48 adult Sprague Dawley rats of either gender and of clean grade, weighing 270-350 g, were included in this study.METHODS: All rats were randomly and evenly divided into 3 groups: experimental, sham-operated, and blank control. In the experimental group, C36 laminectomy for decompression was performed.In the sham-operated group: only skin incision was made. Rats in the blank control group received no any treatment. Four rats were allocated for each time point (1, 2, 4, and 8 weeks post-surgery) for harvesting posterior longitudinal ligament.MAIN OUTCOME MEASURES: Detection of BMP-4 mRNA expression in the cervical posterior longitudinal ligament by semi-quantitative reverse transcription-polymerase chain reaction.RESULTS: In the experimental group, obvious BMP-4 mRNA expression was found at 1-8 weeks post-surgery. While no BMP-4 mRNA expression was found at all times in the sham-operated and blank control groups.CONCLUSION: Posterior cervical decompression can increase BMP-4 mRNA expression in the posterior longitudinal ligament.Endogenous BMP-4 may contribute to ligamentous ossification and development post-surgery.