CAJ | 학술논문

目的探讨黄体生成素受体(LHR)和血管内皮生长因子(VEGF)在围着床期小鼠卵巢中的生物学作用. 方法应用免疫组织化学SABC法和图像分析,检测LHR与VEGF在昆明小鼠( n =28)动情期、孕1d、孕4d和孕6d卵巢中的分布状况和变化规律. 结果 LHR与VEGF免疫阳性细胞具有相似的分布和变化规律.在动情期,较大的卵泡周围的基质细胞呈强阳性着色,与其他组相比,差异显著( P <0.05).随着妊娠的继续,颗粒黄体细胞的着色逐渐增强,在孕6d时表达最强.在动情期和孕1d,部分血管内皮和血细胞分别呈VEGF和LHR强阳性着色.从孕1d开始,卵泡膜细胞呈LHR阳性着色. 结论 LHR和VEGF的表达与围着床期小鼠卵巢中卵泡的发育、排卵和黄体形成过程密切相关.
목적 탐토황체생성소수체(LHR)화혈관내피생장인자(VEGF)재위착상기소서란소중적생물학작용. 방법 응용면역조직화학SABC법화도상분석,검측LHR여VEGF재곤명소서( n =28)동정기、잉1d、잉4d화잉6d란소중적분포상황화변화규률. 결과 LHR여VEGF면역양성세포구유상사적분포화변화규률.재동정기,교대적란포주위적기질세포정강양성착색,여기타조상비,차이현저( P <0.05).수착임신적계속,과립황체세포적착색축점증강,재잉6d시표체최강.재동정기화잉1d,부분혈관내피화혈세포분별정VEGF화LHR강양성착색.종잉1d개시,란포막세포정LHR양성착색. 결론 LHR화VEGF적표체여위착상기소서란소중란포적발육、배란화황체형성과정밀절상관.
Objective To explore the biological effects of the luteinizing hormone receptor (LHR) and vascular endothelial growth factor (VEGF) on the ovary of mice during peri-implantation. Methods The immunohistochemistry SABC method and image analysis were used to study the distribution and changes of the LHR and VEGF in Kunming mouse( n =28) ovary during estrous,pregnancy of day 1, day 4 and day 6 stage. Results The expression of LHR-immunoreactive substance and that of VEGF-immunoreactive substance had the same distribution and changes. Compared with other groups,the level of LHR-immunoreactive substance and that of VEGF-immunoreactive substance increased highly on the stroma cells around largergrowing follicles in estrous group ( P <0.05). Along with the pregnancy, the positive immunostaining for LHR and VEGF increased gradually on the granulosa lutein cells, and reached the highest level on day 6 of pregnancy. Positive immunostaining for LHR or VEGF on some endothelia and blood cells were observed in day 1 of pregnancy or estrous group respectively. Form day 1 of pregnancy, the theca cells had positive immunostaining for LHR. Conclusion The expression of LHR and VEGF is closely related with the process of follicle growing, ovulation and corpus luteum formation.