CAJ | 학술논문

目的评价玻璃化溶液EDFS40对深低温保存兔颈总动脉形态结构和舒缩功能的影响.方法无菌条件下取兔颈总动脉,分别以EDFS40和1.5 mol/L二甲亚砜为低温保护剂,经玻璃化法和慢速冷冻法深低温保存14 d.复温后和新鲜动脉进行大体和显微形态的观察,器官浴槽内进行非内皮依赖性舒缩功能的测定.结果 EDFS40组动脉的大体、组织学及超微结构与新鲜动脉相似,对氯化钾的收缩力和慢速冷冻组动脉无明显差别(P>0.05),对去甲肾上腺素和硝普钠的收缩或舒张张力优于后者(P<0.05),但均低于新鲜动脉(P<0.05).结论 EDFS40能较好保持兔颈总动脉的形态结构及功能,可用于血管的玻璃化保存.
목적 평개파리화용액EDFS40대심저온보존토경총동맥형태결구화서축공능적영향.방법 무균조건하취토경총동맥,분별이EDFS40화1.5 mol/L이갑아풍위저온보호제,경파리화법화만속냉동법심저온보존14 d.복온후화신선동맥진행대체화현미형태적관찰,기관욕조내진행비내피의뢰성서축공능적측정.결과 EDFS40조동맥적대체、조직학급초미결구여신선동맥상사,대록화갑적수축력화만속냉동조동맥무명현차별(P>0.05),대거갑신상선소화초보납적수축혹서장장력우우후자(P<0.05),단균저우신선동맥(P<0.05).결론 EDFS40능교호보지토경총동맥적형태결구급공능,가용우혈관적파리화보존.
Objective To assess the effect of EDFS40 on the structure and vasomotoricity of rabbit carotids in cryopreservation. Methods The fresh carotids, taken from rabbits under aseptic conditions, were preserved for 14 days in liquid nitrogen by vitrification or slow freezing method with both fresh and thawed carotids were observed by visual examination, optical and electron microscopes, respectively. Their endothelium-independent vasoconstriction and vasodilatation were detected in organ bath. Results The morphological changes of the EDFS40-vitrified arteries were similar to those of the fresh carotids under macrography, optical and electron microscopes. And there was no obvious difference in contraction to KCl between the cryopreservation group and the slow-freezing group (P>0.05). The contractile and relaxant responses of vitrified arteries to NE or SNP were significantly better than those of the frozen arteries (P<0.05); both, however, were still inferior to the vasomotoricity of the fresh arteries (P<0.05). Conclusion The rabbit carotids vitrified with EDFS40 can have their structure and vasomotor function maintained without obvious damage, which may indicate the usage of EDFS40 in vitrification of blood vessels.