CAJ | 학술논문

目的研究黄体酮对乳腺癌细胞增殖和迁移的影响.方法 100 nM黄体酮刺激黄体酮受体阳性的乳腺癌细胞株T-47D和MCF-748小时后,用MTT方法检测细胞增殖,用细胞划痕愈合实验检测细胞迁移,用Western blotting检测细胞内E-Cadherin的表达.结果黄体酮刺激MCF-7和T-47D细胞的增殖和迁移;并且可以下调MCF-7和T-47DE-Cadherin的表达.结论黄体酮能够促进培养乳腺癌细胞的增殖和迁移,提示抗黄体酮治疗可能有临床意义.
목적 연구황체동대유선암세포증식화천이적영향.방법 100 nM황체동자격황체동수체양성적유선암세포주T-47D화MCF-748소시후,용MTT방법 검측세포증식,용세포화흔유합실험검측세포천이,용Western blotting검측세포내E-Cadherin적표체.결과 황체동자격MCF-7화T-47D세포적증식화천이;병차가이하조MCF-7화T-47DE-Cadherin적표체.결론 황체동능구촉진배양유선암세포적증식화천이,제시항황체동치료가능유림상의의.
Objective To investigate the effects of progesterone on the growth and migration of breast cancer cells, Methods MCF-7 and T-47D cells were cultured in DMEM and stimulated with 100 nmol/L progesterone for 48 h, and the cell proliferation was evaluated by MTT assay, cell migration by wound-healing assay and E-catherin expression by Western blotting. Results Progesterone stimulated the cell proliferation and migration and down-regulated the expression of E-catherin in both MCF-7 and T-47D cells. Conclusions Progesterone stimulates the cell proliferation and migration of cultured breast cancer cells, suggesting the clinical significance of anti-progesterone therapy in breast cancer.