CAJ | 학술논문

研究了6种多氯联苯(PCBs)3,3',4,4'-四氯联苯(PCB 77)、2,3,3',4,4'-五氯联苯(PCB 105)、2,3',4,4',5-五氯联苯(PCB 118)、3,3',4,4',5-五氯联苯(PCB 126)、2,3,3',4,4',5-六氯联苯(PCB 156)和商业型混合多氯联苯Aroclor 1254,两种多溴联苯醚(PBDEs)2,2',4,4'-四溴二苯醚(PBDE 47)、十溴二苯醚(PBDE 209)对人类癌细胞生长和斑马鱼脱膜与不脱膜胚胎发育的影响.8种化合物均使用0.01、0.1、1.0、10μmol·L~(-1)4个浓度进行1-6d的暴露实验.结果表明,PBDE 209在最高浓度10μmol·L~(-1)下对结肠癌细胞HCT116(暴露3d后)和RKO(暴露5d后)具有显著的生长抑制作用,所有化合物均对乳腺癌细胞没有显著影响.相比之下,化合物对受精后5-6b(5～6hpf)的斑马鱼胚胎的毒性效应显得比较明显,而各化合物对胚胎的致畸和致死效应又不相同,其毒性强弱依次为PCB 126≈PCB 156>PCB 1254(Aroclor 1254)>PBDE 47>PCB 77>PCB 105≈PCB 118≈PBDE 209.其中PBDE 209在未脱膜暴毒后均无致畸与致死现象,脱膜暴毒后最高浓度才表现出显著意义的致畸作用,而PBDE 47在最高浓度下可产生高达80%的致畸率,这说明胚胎绒毛膜具有有效阻挡大分子物质如PBDE 209进入的作用.PCBs的毒性效应与其空间结构密切相关.如PCB 126和PCB 105具有相同的分子式,前者在1μmol·L~(-1)下就引起了显著的致死和致畸效应,而后者即使在10μmol·L~(-1)下也没有显著的效应.实验结果也说明不同类型的实验对象所展示的毒性效应并不相同,化合物对体外培养的细胞和发育中的胚胎具有不同的影响.
연구료6충다록련분(PCBs)3,3',4,4'-사록련분(PCB 77)、2,3,3',4,4'-오록련분(PCB 105)、2,3',4,4',5-오록련분(PCB 118)、3,3',4,4',5-오록련분(PCB 126)、2,3,3',4,4',5-륙록련분(PCB 156)화상업형혼합다록련분Aroclor 1254,량충다추련분미(PBDEs)2,2',4,4'-사추이분미(PBDE 47)、십추이분미(PBDE 209)대인유암세포생장화반마어탈막여불탈막배태발육적영향.8충화합물균사용0.01、0.1、1.0、10μmol·L~(-1)4개농도진행1-6d적폭로실험.결과표명,PBDE 209재최고농도10μmol·L~(-1)하대결장암세포HCT116(폭로3d후)화RKO(폭로5d후)구유현저적생장억제작용,소유화합물균대유선암세포몰유현저영향.상비지하,화합물대수정후5-6b(5～6hpf)적반마어배태적독성효응현득비교명현,이각화합물대배태적치기화치사효응우불상동,기독성강약의차위PCB 126≈PCB 156>PCB 1254(Aroclor 1254)>PBDE 47>PCB 77>PCB 105≈PCB 118≈PBDE 209.기중PBDE 209재미탈막폭독후균무치기여치사현상,탈막폭독후최고농도재표현출현저의의적치기작용,이PBDE 47재최고농도하가산생고체80%적치기솔,저설명배태융모막구유유효조당대분자물질여PBDE 209진입적작용.PCBs적독성효응여기공간결구밀절상관.여PCB 126화PCB 105구유상동적분자식,전자재1μmol·L~(-1)하취인기료현저적치사화치기효응,이후자즉사재10μmol·L~(-1)하야몰유현저적효응.실험결과야설명불동류형적실험대상소전시적독성효응병불상동,화합물대체외배양적세포화발육중적배태구유불동적영향.
Six polychlorinated biphenyl (PCB) congeners,one technical PCB mixture,and two polybrominated diphenyl ether (PBDE) congeners were studied for their cytotoxicity on four human cancer cell lines and developmental toxicity on zebrafish embryos with or without chorions.All chemicals were tested at four concentrations for exposure time periods ranging from 1 to 6 days.The findings showed no adverse effects on cell growth for any chemicals except that high concentrations of PBDE 209 caused significant inhibition of proliferation in two Human Colon Cancer cell lines,RKO and HCT116,after 3-6 day exposure.PBDE 209 had no effect on Breast Cancer Cells ZR75-1 and MDA-MB-231.In contrast,continuous exposure of zebralish embryos beginning at 5-6 hour post-fertilization to PCBs and PBDEs fbr 6 days produced concentration-dependent increases in malformation and mortality.The degree of sensitivity varied with congener and the presence of a chorion.Specifically,PBDE 209 showed no adverse effect on zebrafish embryos with intact chorions while this chemical led to malformation in 43.33% of larvae without chorions at the highest concentration and exposure time period,suggesting chorions act as barriers for high molecular weight chemicals such as PBDE 209.The PCB structure also influenced the embryonic response.For example,PCB 126 induced severe developmental toxicity at 1μmol·L~(-1) in embryos with or without chorions,while PCB 105 produced no observable effects even at 10μmol·L~(-1).The developmental toxicity of these chemicals in descending order is PCB 126≈PCB 156>PCB 1254(Aroclor1254)>PBDE 47>PCB 77>PCB 105≈PCB 118≈PBDE 209.The findings indicate that the toxic response varies between individual cells and in developing embryos where cellular complexity is very different.