CAJ | 학술논문

背景:生长因子能促进侧支血管的发育,且多种因子协同效果更为明显,骨髓液中富含多种生长因子.目的:观察血管内膜损伤后的骨髓液对鸡胚绒毛尿囊膜血管生成的作用.方法:受精蛋70枚在(37.5±0.5) ℃条件下孵育,第7天开窗,第8天将存活鸡胚随机分为生理盐水组、正常血清组、正常骨髓液组、损伤血清组、损伤骨髓液组以及血管内皮生成因子组,每组10枚,分别滴加5 μL兔正常血清、5 μL兔骨髓液、5 μL兔血管内膜损害血清、5 μL兔血管内膜损害骨髓液、5 μL生理盐水及0.3 μg 血管内皮生长因子进鸡胚绒毛尿囊膜中,连续3 d.数码相机拍照后平铺于载玻片上,计数鸡胚绒毛尿囊膜新生的血管数目.结果与结论:与正常血清组相比,正常骨髓液组、血管内膜损害血清组鸡胚绒毛尿囊膜新生的血管总数明显增多,大中血管明显增生;且血管内膜损害血清组大、中血管数更为明显增加.提示正常骨髓液具有明显的促进鸡胚绒毛尿囊膜模型血管生成的作用,其强度优于血管内皮生长因子;血管内膜损伤第7天的血清和骨髓液能够明显的促进鸡胚绒毛尿囊膜上的血管生成,其强度优于血管内皮生长因子组.
배경:생장인자능촉진측지혈관적발육,차다충인자협동효과경위명현,골수액중부함다충생장인자.목적:관찰혈관내막손상후적골수액대계배융모뇨낭막혈관생성적작용.방법:수정단70매재(37.5±0.5) ℃조건하부육,제7천개창,제8천장존활계배수궤분위생리염수조、정상혈청조、정상골수액조、손상혈청조、손상골수액조이급혈관내피생성인자조,매조10매,분별적가5 μL토정상혈청、5 μL토골수액、5 μL토혈관내막손해혈청、5 μL토혈관내막손해골수액、5 μL생리염수급0.3 μg 혈관내피생장인자진계배융모뇨낭막중,련속3 d.수마상궤박조후평포우재파편상,계수계배융모뇨낭막신생적혈관수목.결과여결론:여정상혈청조상비,정상골수액조、혈관내막손해혈청조계배융모뇨낭막신생적혈관총수명현증다,대중혈관명현증생;차혈관내막손해혈청조대、중혈관수경위명현증가.제시정상골수액구유명현적촉진계배융모뇨낭막모형혈관생성적작용,기강도우우혈관내피생장인자;혈관내막손상제7천적혈청화골수액능구명현적촉진계배융모뇨낭막상적혈관생성,기강도우우혈관내피생장인자조.
BACKGROUND: Growth factor can promote the development of collateral vessels. The synergistic effects of multiple factors are obvious. Many kinds of growth factors are enriched in bone marrow serum. OBJECTIVE: To determine effects of bone marrow serum on angiogenesis in chick chorioallantoic membranes following vascular intimal injury. METHODS: A total of 70 fertilized chick eggs were incubated at (37.5±0.5) ℃ and made a window in every egg at day 7. The survival chick embryos were randomly divided into six groups: normal saline group, normal blood serum group, normal bone marrow serum group, blood serum of rabbit suffered with vascular intimal injury group, bone marrow serum of rabbit suffered with vascular intimal injury group and vascular endothelial growth factor group (n=10), which were respectively treated with 5 μL rabbit normal blood serum, 5 μL rabbit normal bone marrow serum, 5 μL blood serum of rabbit suffered with vascular intimal injury, 5 μL bone marrow serum of rabbit suffered with vascular intimal injury, 5 μL normal saline and 0.3 μg vascular endothelial growth factor in chick chorioallantoic membranes, for 3 consecutive days. Pictures were taken by a digital camera, and the total number of the vessels around the disc was measured. RESULTS AND CONCLUSION: The total numbers of vessels in normal bone marrow serum group and blood serum of rabbit suffered with vascular intimal injury group were obviously higher than that of normal blood serum group. Large and middle vessels were obviously proliferated. Large and middle vessels were more observed in the blood serum of rabbit suffered with vascular intimal injury group. These indicated that normal bone marrow serum can stimulate angiogenesis in models of chick chorioallantoic membranes. Compared with vascular endothelial growth factor, both the normal bone serum and the bone marrow serum of rabbit suffered from vascular intimal injury were more effective in angiogenesis. The blood serum and bone marrow serum from vascular intimal injured rabbit at day 7 can significantly promote angiogenesis in chick chorioallantoic membranes, which was better than the vascular endothelial growth factor group.