CAJ | 학술논문

目的研究芍药甘草汤提取物芍甘多苷对四氯化碳(CC14)亚急性肝损伤大鼠血清转氨酶、肝脏病理及肝脏基因表达谱的影响.方法 60只SD大鼠完全随机分6组(n=10),即正常组、模型组、阳性对照组(联苯双酯100 mg/kg)和芍甘多苷低、中、高3个剂量组(88、264、528 mg/kg).采用CCl4诱发大鼠亚急性肝损伤,然后给予不同剂量的芍甘多苷灌胃,观察药物对血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肝脏组织病理的影响；并运用基因表达谱芯片技术,研究其对肝脏基因表达谱的影响.结果与模型组比较,芍甘多苷高、中剂量灌胃给药均能显著降低CCl4亚急性肝损伤大鼠血清ALT[ (218.37± 114.59,234.59± 109.33) U/L比(389.12±142.57) U/L,P＜0.05]；且高剂量芍甘多苷可以显著降低肝脏组织学评分(2.42±0.52比3.92±0.84,P＜0.05).芍甘多苷能上调和(或)下调与肝损伤相关的多种基因的表达.结论芍甘多苷有明显的肝脏保护作用,其机制与调节与肝损伤相关的多种基因的表达有关,是多靶点作用药物.
목적 연구작약감초탕제취물작감다감대사록화탄(CC14)아급성간손상대서혈청전안매、간장병리급간장기인표체보적영향.방법 60지SD대서완전수궤분6조(n=10),즉정상조、모형조、양성대조조(련분쌍지100 mg/kg)화작감다감저、중、고3개제량조(88、264、528 mg/kg).채용CCl4유발대서아급성간손상,연후급여불동제량적작감다감관위,관찰약물대혈청병안산전안매(ALT)、천동안산전안매(AST)、간장조직병리적영향；병운용기인표체보심편기술,연구기대간장기인표체보적영향.결과 여모형조비교,작감다감고、중제량관위급약균능현저강저CCl4아급성간손상대서혈청ALT[ (218.37± 114.59,234.59± 109.33) U/L비(389.12±142.57) U/L,P＜0.05]；차고제량작감다감가이현저강저간장조직학평분(2.42±0.52비3.92±0.84,P＜0.05).작감다감능상조화(혹)하조여간손상상관적다충기인적표체.결론 작감다감유명현적간장보호작용,기궤제여조절여간손상상관적다충기인적표체유관,시다파점작용약물.
Objective To investigate influences of effective components of paeornia and glycyrrhiza decoction (Shao Gan Duo Gan) on serum transaminase,liver pathology and liver gene expression profile in rats with subacute liver injury induced by carbon tetrachloride.Methods Sixty SD rats were randomized into 6 groups:normal,model,positive control (biphenyl dimethylester 100 mg/kg),and 3 Shao Gan Duo Gan treatment groups (with doses of 88,264,528 mg/kg).Subacute liver injury was induced by carbon tetrachloride in the rats which were then treated by intragastric administration of different doses of Shao Gan Duo Gan.The effects of different treatments on serum ALT,AST and liver pathology were observed,and gene expression chips were used to observe changes in gene expression profile of the liver.Results Compared to model group,intragastric administration of high and intermediate doses of Shao Gan Duo Gan remarkably reduced the serum ALT in the injured rats [ (218.37±114.59,234.59±109.33) U/L vs (389.12±142.57) U/L,P＜0.05].The high dose of Shao Gan Duo Gan significantly decreased the histologic scores of the liver (2.42±0.52 vs 3.92±0.84,P＜0.05),improved degeneration and necrosis of the hepatocytes,and upregulated or down-regulated expressions of genes related to liver jnjury.Conclusion Shao Gan Duo Ganhas an obvious liver protection,probably because it can regulate expressions of multiple genes related to the liver injury.It may be a drug which has an effect on multiple targets.