CAJ | 학술논문

目的探讨Survivin、Smad4/dpo4、APC基因在大肠腺瘤、腺癌中的表达及意义.方法采用免疫组化SP法检测40例正常大肠组织、80例腺瘤、80例腺癌中三种基因表达情况.结果 Survivin基因在正常大肠组织、腺瘤、腺癌中阳性表达率分别为0、35.0%、75.0%;Smad4基因为100%、95.0%、78.8%;APC为100%、80.0%、45.0%.结论 Survivin基因检测可以作为大肠癌早期诊断、观察疗效和监测肿瘤术后复发、转移等生物学行为的一个新指标.Smad4基因突变或缺失不仅诱导大肠癌的发生还促进其发展.检测APC基因突变,有助于对肿瘤病因学、发病机制及早期诊断的研究.
목적 탐토Survivin、Smad4/dpo4、APC기인재대장선류、선암중적표체급의의.방법 채용면역조화SP법검측40례정상대장조직、80례선류、80례선암중삼충기인표체정황.결과 Survivin기인재정상대장조직、선류、선암중양성표체솔분별위0、35.0%、75.0%;Smad4기인위100%、95.0%、78.8%;APC위100%、80.0%、45.0%.결론 Survivin기인검측가이작위대장암조기진단、관찰료효화감측종류술후복발、전이등생물학행위적일개신지표.Smad4기인돌변혹결실불부유도대장암적발생환촉진기발전.검측APC기인돌변,유조우대종류병인학、발병궤제급조기진단적연구.
Objective To observe the solo-allied expression of Survivin, Smad4/dpc4 and APC gene, and study the relationship between the three genes and biological behavior of colorectal cancer. Methods The expressions of Survivin, Smad4/dpc4 and APC gene were detected by SP immunohistochem-istry among the cases from 40 eu-intestine ,80 large intestinal adenoma and 80 CRC. Results The positive rate of survivin protein in the eu-intestine, the large intestinal adenoma and CRC was 0,35.0% ,75.0% , respectively. The positive rate of Smad4/dpc4 protein was 100% , 95. 0% , 78. 8% , and the positive rate of APC protein was 100% ,80.0% ,45.0% , respectively. In the eu-intestine, APC + Smad4/dpc4 Z- expressed in 40 cases, with the incidence of 100%. In the large intestinal adenoma, APC + Smad4 expressed in 38 patients, with the incidence of 47. 5% , and the three genes expressed in 29 patients with the incidence of 23. 8%. In the CRC, two genes expressed in 57 patients, with the incidence of 71. 3% , and three genes expressed in 28 patients, with the incidence of 35.0%. Conclusion The detection of survivin was a new indicator in early diagnosis of CRC. It was significant in the diagnosis of CRC that energetic search for the positive rate level realm of survivin. The catastrophe or absence of Smad4/dpc4 gene not only induced the genesis of CRC but also encouraged its growth. Smad4/dpc4 was ant- carcinomatous gene intimately correlated to the CRC. Detection of APC gene had very important significance, it could be helpful to conduct the research of tumorous aetiology, nosogenesis and early diagnosis. The allied detection of Survivin, Smad4/dpc4, APC gene played a very important role in early diagnosing and healing the CRC.