CAJ | 학술논문

辽宁省部分地区2006年虫媒病毒分离鉴定
요녕성부분지구2006년충매병독분리감정
Isolation and identification of arboviruses from mosquito pools in some regions of Liaoning province,China

万方数据

中华流行病学杂志中華流行病學雜誌 중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年 1期 50-54 ,共5页

孟维珊%张稷博%孙肖红%刘起男%陈哲%翟友刚%付士红%曹玉玺%王环宇%丁俊%褚发俊%李志%张立田%赵玉军%梁国栋

맹유산%장직박%손초홍%류기남%진철%적우강%부사홍%조옥새%왕배우%정준%저발준%리지%장립전%조옥군%량국동

虫媒病毒%版纳病毒%甲病毒%序列分析%种系发生蟲媒病毒%版納病毒%甲病毒%序列分析%種繫髮生
충매병독%판납병독%갑병독%서렬분석%충계발생
Arboviruses%Banna virus%Getah virus%Sequencing analysis%Phylogeny

目的调查辽宁省虫媒病毒的种类及分布.方法 2006年8月在辽宁省沈阳市、营口市、盘锦市、锦州市和丹东市采集蚊虫标本,利用组织细胞培养分离病毒,对病毒分离物进行血清学和分子生物学鉴定.结果 5个市共采集蚊虫标本5410只,分离到8株阳性分离物,经鉴定其中3株(LN0684、LN0688、LN0689)为版纳病毒,1株(LN0636)为甲病毒属盖塔病毒,另外4株尚在鉴定.新分离的版纳病毒与我国此前的分离株处于同一个进化簇,核苷酸同源性91.2%～94.7%.新分离的盖塔病毒与韩国分离株(swine)在一个进化枝上,核苷酸同源性为99.2%,与俄罗斯分离株、中国大陆分离株及台湾分离株的核苷酸同源性在95%～99%之间.结论 2006年在辽宁省分离到3株版纳病毒、1株盖塔病毒和4株未知虫媒病毒.版纳病毒、盖塔病毒为辽宁省首次分离;盖塔病毒核苷酸同源性和韩国分离株最近.
목적 조사요녕성충매병독적충류급분포.방법 2006년8월재요녕성침양시、영구시、반금시、금주시화단동시채집문충표본,이용조직세포배양분리병독,대병독분리물진행혈청학화분자생물학감정.결과 5개시공채집문충표본5410지,분리도8주양성분리물,경감정기중3주(LN0684、LN0688、LN0689)위판납병독,1주(LN0636)위갑병독속개탑병독,령외4주상재감정.신분리적판납병독여아국차전적분리주처우동일개진화족,핵감산동원성91.2%～94.7%.신분리적개탑병독여한국분리주(swine)재일개진화지상,핵감산동원성위99.2%,여아라사분리주、중국대륙분리주급태만분리주적핵감산동원성재95%～99%지간.결론 2006년재요녕성분리도3주판납병독、1주개탑병독화4주미지충매병독.판납병독、개탑병독위요녕성수차분리;개탑병독핵감산동원성화한국분리주최근.
Objective To isolate and identify arboviruses from mosquito pools in some regions of Liaoning province.Methods Mosquitoes were collected from Shenyang,Yingkou,Panjin,Jinzhou and Dandong cities of Liaoning province in 2006.Viruses were isolated by inoculating the specimens onto C6/ 36 and BHK-21cells.The new isolates were identified using serological and molecular biological methods.Results 5410 mosquitoes were collected from the five cities in total.Three isolates produced CPE in C6/ 36 cell and five isolates produced CPE in both C6/36 and BI-IK-21 cell.Three isolates (LN0684,LN0688 and LN0689) were identified as Banna virus and one isolate (LN0636) was identified as Getah virus.Phylogenetic analysis showed that the three Banna virus strains were clustered into the same evolution branch as the other Chinese isolates.The identity of nucleotide sequence was between 91.2% and 94.7%,compared with other Banna virus strains.The new isolated Getah virus was clustered into the same branch with the strain of South Korea (swine).The identity of nucleotide sequence was 99.2%,when comparing with the strain of South Korea and was 95% to 99% with the strains fi'om Russia,mainland of China and Taiwan region.Conehmion Eight virus isolates,including three Banna virus,one Getah virus and four unknown virus strains were isolated from mosquitoes in Liaoning province.Banna virus and Getah virus were reported for the first time in Liaoning province,while Getah virus showed the highest nucleotide homology with the South Korea strains.