CAJ | 학술논문

目的探讨白藜芦醇对中波紫外线(UVB)照射致体外培养人皮肤成纤维细胞氧化损伤的预防和治疗作用.方法实验分为5组:正常对照组、白藜芦醇组、UVB照射组、UVB照射后白藜芦醇处理组及白藜芦醇预处理后UVB照射组.细胞处理后用甲基噻唑基四唑(MTT)法检测细胞增殖活性,酶生化法检测细胞超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量.结果白藜芦醇浓度>100μmol/L时可抑制成纤维细胞增殖,<100 μmol/L时可促进成纤维细胞增殖,最佳作用浓度为50μmol/L;UVB照射后细胞活性下降31.7%,细胞SOD活性降低、MDA产生增加;与单纯UVB照射相比,UVB照射前或照射后用白藜芦醇处理均能使成纤维细胞存活率提高、SOD活性增强、MDA产生减少.结论低浓度白藜芦醇可促进成纤维细胞增殖,从而对因UVB照射而损伤的体外培养成纤维细胞起到保护作用.
목적 탐토백려호순대중파자외선(UVB)조사치체외배양인피부성섬유세포양화손상적예방화치료작용.방법 실험분위5조:정상대조조、백려호순조、UVB조사조、UVB조사후백려호순처리조급백려호순예처리후UVB조사조.세포처리후용갑기새서기사서(MTT)법검측세포증식활성,매생화법검측세포초양화물기화매(SOD)활성、병이철(MDA)함량.결과 백려호순농도>100μmol/L시가억제성섬유세포증식,<100 μmol/L시가촉진성섬유세포증식,최가작용농도위50μmol/L;UVB조사후세포활성하강31.7%,세포SOD활성강저、MDA산생증가;여단순UVB조사상비,UVB조사전혹조사후용백려호순처리균능사성섬유세포존활솔제고、SOD활성증강、MDA산생감소.결론 저농도백려호순가촉진성섬유세포증식,종이대인UVB조사이손상적체외배양성섬유세포기도보호작용.
Objective To explore the protective effect of resveratrol against oxidative damage to cultivated fibroblasts irradiated with UVB. Methods Fibroblasts from normal human skin cultured in vitro were divided into 5 groups (a normal control group, a group irradiated with UVB, a group treated with resveratrol before UVB irradiation, and a group treated after irradiation). A monolayer of fibroblasts was irradiated with UVB at 60 mJ/cm2. The vitality of the cells was measured using the methylthiazol tetrazolium (MTT) method. The activity of superoxide dis-mutase (SOD) and malondialdehyde (MDA) content were determined using enzyme biochemistry. Results Resveratrol over 100 μM inhibited the proliferation of fibroblasts. Resveratrol under 100 μM improved the proliferation of cells. The optimal concentration was 50 μM. UVB irradiation decreased the vitality of the cells and SOD activity, and it significantly enhanced MDA content. Conclusions Resveratrol treatment before or after UVB irradiation elevates the survival rate of fibroblasts, enhances the activity of SOD, and decreases MDA content. Resveratrol at low concentration could improve the proliferation of fibroblasts, and at high concentration could inhibit their proliferation. Res-veratol at 50 μM relieves the inhibited proliferation of fibroblasts damaged by UVB irradiation.