CAJ | 학술논문

目的探讨纳米二氧化钛灌胃染毒对胃溃疡大鼠血象的影响.方法对纳米二氧化钛材料进行表征.将24只8周龄清洁级雄性SD大鼠按体重用随机数字表法分为4组(每组6只).在胃体部与幽门窦交界处注射体积分数为20％的醋酸,建立胃溃疡模型后,每天1次灌胃,分别给予0(对照组)、10(低剂量组)、50(中剂量组)、200 mg/kg(高剂量组)纳米二氧化钛,染毒30 d后进行血液常规指标和凝血指标的检测,并分析其变化情况.结果纳米二氧化钛为锐钛矿晶型,近球形,平均粒径( 75±15)nm.高剂量纳米二氧化钛染毒组大鼠的广细胞(WBC)计数[(8.48±3.28)×109/L]、淋巴细胞(LYM)计数[(6.85±2.53)×109/L]、单核细胞(MOD)计数[(0.27±0.12)×109/L]、中性粒细胞(GRN)计数[(1.37±0.86)×109/L]、红细胞(RBC)计数[(8.20 ±0.49)×109/L]、红细胞压积(HCT)[(45.3±1.4)％]均高于对照组[分别为(2.63±0.34)×109/L、(2.25±0.26)×109/L、(0.05±0.06)×109/L、(0.33±0.26)×109/L、(4.87±2.37)×109/L、(27.2±13.3)％],差异有统计学意义(t值分别为-3.449、-3.825、-3.554、-3.097、-2.972、-2.936,P值均＜0.05)；中剂量组大鼠的WBC[ (6.88 ±3.06)×109/L]、MOD[ (0.20±0.07)×109/L]、RBC [(7.79±0.48)×109/L]、HCT[ (42.7±2.8)％]亦明显高于对照组,差异有统计学意义(t值分别为-2.507、-2.367、-2.605、-2.511,P值均＜0.05).各染毒组大鼠与对照组相比,其他血常规和凝血相关检测指标未见明显改变.结论较长期经口摄入纳米二氧化钛,可导致胃溃疡大鼠WBC和RBC计数指标的明显升高,但对血小板和凝血相关指标无明显影响.
목적 탐토납미이양화태관위염독대위궤양대서혈상적영향.방법 대납미이양화태재료진행표정.장24지8주령청길급웅성SD대서안체중용수궤수자표법분위4조(매조6지).재위체부여유문두교계처주사체적분수위20％적작산,건립위궤양모형후,매천1차관위,분별급여0(대조조)、10(저제량조)、50(중제량조)、200 mg/kg(고제량조)납미이양화태,염독30 d후진행혈액상규지표화응혈지표적검측,병분석기변화정황.결과 납미이양화태위예태광정형,근구형,평균립경( 75±15)nm.고제량납미이양화태염독조대서적엄세포(WBC)계수[(8.48±3.28)×109/L]、림파세포(LYM)계수[(6.85±2.53)×109/L]、단핵세포(MOD)계수[(0.27±0.12)×109/L]、중성립세포(GRN)계수[(1.37±0.86)×109/L]、홍세포(RBC)계수[(8.20 ±0.49)×109/L]、홍세포압적(HCT)[(45.3±1.4)％]균고우대조조[분별위(2.63±0.34)×109/L、(2.25±0.26)×109/L、(0.05±0.06)×109/L、(0.33±0.26)×109/L、(4.87±2.37)×109/L、(27.2±13.3)％],차이유통계학의의(t치분별위-3.449、-3.825、-3.554、-3.097、-2.972、-2.936,P치균＜0.05)；중제량조대서적WBC[ (6.88 ±3.06)×109/L]、MOD[ (0.20±0.07)×109/L]、RBC [(7.79±0.48)×109/L]、HCT[ (42.7±2.8)％]역명현고우대조조,차이유통계학의의(t치분별위-2.507、-2.367、-2.605、-2.511,P치균＜0.05).각염독조대서여대조조상비,기타혈상규화응혈상관검측지표미견명현개변.결론 교장기경구섭입납미이양화태,가도치위궤양대서WBC화RBC계수지표적명현승고,단대혈소판화응혈상관지표무명현영향.
Objective To explore the effect of titanium dioxide ( TiO2 ) nanoparticles on hemogram in rats with gastric ulcer.Methods Physicochemical properties of TiO2 nanoparticles were characterized.Twenty-four clear class SD male rats,aging 8 week-old,were randomly divided into 4 groups,6 rats for each group.20％ acetic acid were injected into the rats' stomach on the border of gastric body and pyloric antrum,and herebv established the gastric ulcer model. The rats in 4 groups were exposed to TiO2 nanoparticles through intragastric administration at 0,10,50 and 200 mg/kg body weight respectively for 30 days. Afterwards, the rats were conducted blood routine test and blood coagulation test for analysis.Results TiO2 nanoparticles were anatase crystals,closely spherical shape,whose average grain diameter was (75 ± 15) nm. The levels of white blood cell (WBC) count ( (8.48 ±3.28) × 109/L),lymphocyte ( LYM ) ( (6.85 ±2.53) × 109/L),moncyte (MOD) ( (0.27 ±0.12) × 109/L),granulocyte (GRN) ((1.37 ±0.86) × 109/L),red blood cell (RBC) ((8.20 ±0.49) × 109/L) and hematocrit (HCT) ( (45.3 ± 1.4 )％ ) in the 200 mg/kg dose group were significantly higher than those in the control group ((2.63±0.34) × 109/L,(2.25 ±0.26) × 109/L,(0.05 ±0.06) × 109/L,(0.33 ±0.26) ×109/L,(4.87 ± 2.37) × 109/L and (27.2± 13.3)％,respectively;t values were - 3.449,- 3.825,-3.554,-3.097,-2.972 and -2.936 respectively,P values all ＜0.05).The levels of WBC( (6.88 ±3.06) ×109/L),MOD ((0.20±0.07) ×109/L),RBC ((7.79±0.48) × 109/L) and HCT ((42.7±2.8 )％ ) in 50 mg/kg dose group were also statistically higher than those in the control group (t values were - 2.507,- 2.367,- 2.605 and - 2.511 respectively,all P values ＜ 0.05 ).There was no statistically difference found in other blood routine index and coagulation index between the three experimental groups and control group.Conclusion The long term intake of TiO2 nanoparticles caused a statistically increase in the amount of WBC and RBC in rats with gastric ulcer; however,there was no obvious changes found in blood platelet and coagulation index.