中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2011年
3期
41-43
,共3页
溃疡性结肠炎%白细胞介素-6受体%白细胞介素-8受体
潰瘍性結腸炎%白細胞介素-6受體%白細胞介素-8受體
궤양성결장염%백세포개소-6수체%백세포개소-8수체
Ulcerative colitis%Interleukin-6 receptor%Interleukin-8 receptor
目的 探讨溃疡性结肠炎(UC)大鼠结肠黏膜白细胞介素-6(IL-6)受体及白细胞介素-8(IL-8)受体的表达变化.方法 将雄性SD大鼠36只完全随机分为正常组、模型1组、模型2组共3 组,每组12只.用5%2,4,6-三硝基苯磺酸100 mg/kg灌肠建立大鼠溃疡性结肠炎模型,7 d后处死模型1组动物,14 d后处死模型2组动物.运用Real-Time-PCR法、ELISA法分别检测各组大鼠IL-6受体及IL-8受体 mRNA及其含量的表达.结果 模型1组、2组IL-6受体mRNA及含量表达分别为(3.35±0.12)pg/ml、(111.35±25.09)pg/ml及(3.56±0.09)pg/ml、(160.11±15.54)pg/ml;模型1组、2组IL-8受体 mRNA及含量表达分别为(3.50±0.19)pg/ml、(160.11±15.54)pg/ml及(3.68±0.16)pg/ml、(185.38±15.42)pg/ml,与正常组相比,差异有统计学意义(P<0.01).结论 活动性UC在炎症诱导下结肠组织中IL-6 受体及IL-8受体 mRNA及其含量表达增高,IL-6 受体及IL-8受体的水平与肠道炎症程度呈正相关.
目的 探討潰瘍性結腸炎(UC)大鼠結腸黏膜白細胞介素-6(IL-6)受體及白細胞介素-8(IL-8)受體的錶達變化.方法 將雄性SD大鼠36隻完全隨機分為正常組、模型1組、模型2組共3 組,每組12隻.用5%2,4,6-三硝基苯磺痠100 mg/kg灌腸建立大鼠潰瘍性結腸炎模型,7 d後處死模型1組動物,14 d後處死模型2組動物.運用Real-Time-PCR法、ELISA法分彆檢測各組大鼠IL-6受體及IL-8受體 mRNA及其含量的錶達.結果 模型1組、2組IL-6受體mRNA及含量錶達分彆為(3.35±0.12)pg/ml、(111.35±25.09)pg/ml及(3.56±0.09)pg/ml、(160.11±15.54)pg/ml;模型1組、2組IL-8受體 mRNA及含量錶達分彆為(3.50±0.19)pg/ml、(160.11±15.54)pg/ml及(3.68±0.16)pg/ml、(185.38±15.42)pg/ml,與正常組相比,差異有統計學意義(P<0.01).結論 活動性UC在炎癥誘導下結腸組織中IL-6 受體及IL-8受體 mRNA及其含量錶達增高,IL-6 受體及IL-8受體的水平與腸道炎癥程度呈正相關.
목적 탐토궤양성결장염(UC)대서결장점막백세포개소-6(IL-6)수체급백세포개소-8(IL-8)수체적표체변화.방법 장웅성SD대서36지완전수궤분위정상조、모형1조、모형2조공3 조,매조12지.용5%2,4,6-삼초기분광산100 mg/kg관장건립대서궤양성결장염모형,7 d후처사모형1조동물,14 d후처사모형2조동물.운용Real-Time-PCR법、ELISA법분별검측각조대서IL-6수체급IL-8수체 mRNA급기함량적표체.결과 모형1조、2조IL-6수체mRNA급함량표체분별위(3.35±0.12)pg/ml、(111.35±25.09)pg/ml급(3.56±0.09)pg/ml、(160.11±15.54)pg/ml;모형1조、2조IL-8수체 mRNA급함량표체분별위(3.50±0.19)pg/ml、(160.11±15.54)pg/ml급(3.68±0.16)pg/ml、(185.38±15.42)pg/ml,여정상조상비,차이유통계학의의(P<0.01).결론 활동성UC재염증유도하결장조직중IL-6 수체급IL-8수체 mRNA급기함량표체증고,IL-6 수체급IL-8수체적수평여장도염증정도정정상관.
Objective To discuss the expression and significance of IL-6 receptor and IL-8 receptor in a rat model of ulcerative colitis. Methods Thirty-six SD rats were randomly divided into 3 groups, twelve rats in each group. group 1:normal; group 2:blank 1; group 3:blank 2.The rat model of ulcerative colitis was brought about by an enema of 100 mg/kg of TNBS and 5% ethanol. The blank 1 rat was sacrificed 1 week later. The other rats were sacrificed 2 weeks later. The expression of IL-6R and IL-8R were detected with the technique of ELISA and Real-Time PCR. Results The mRNA and protein expression of IL-6R were (3.35±0.12)pg/ml,(111.35±25.09)pg/ml and (3.56±0.09)pg/ml,(160.11±15.54)pg/ml in blank 1 and blank 2.While the mRNA and protein expression of IL-8R were(3.50±0.19)pg/ml,(160.11±15.54)pg/ml and(3.68±0.16)pg/ml,(185.38±15.42)pg/ml respectively. Compared to normal group, there was a significant difference in the results of CINC-1 and CXCR2 (P<0.01). Conclusions The mRNA and protein expression of IL-6R and IL-8R is on the increment in active UC.There is relation between UC and the expression of IL-6R and IL-8R.
