CAJ | 학술논문

目的研究人参皂苷Rg1对铅引起的大鼠海马CA1区长时程增强(LTP)的损伤是否有修复作用.方法 Wistar大鼠从出生至断奶通过母乳摄入铅(母鼠每天饮用0.2%醋酸铅溶液20 mL),在出生后的20～25 d记录其海马CA1区兴奋性突触后电位并诱导长时程增强.结果人参皂苷Rg1 (50 μmol·L-1)灌流对照组和铅暴露组大鼠海马脑片20 min均能诱导出LTP,铅暴露组大鼠的LTP幅度较对照组低.在铅暴露组大鼠的海马脑片上,高频刺激(HFS,1 s, 100 Hz)诱导的LTP较对照组显著降低, 50 μmol·L-1人参皂苷Rg1灌流20 min,HFS 诱导的LTP幅度提高47.1%.结论人参皂苷Rg1能够提高基础的突触传递,并能部分修复铅损伤的HFS-LTP.
목적 연구인삼조감Rg1대연인기적대서해마CA1구장시정증강(LTP)적손상시부유수복작용.방법 Wistar대서종출생지단내통과모유섭입연(모서매천음용0.2%작산연용액20 mL),재출생후적20～25 d기록기해마CA1구흥강성돌촉후전위병유도장시정증강.결과 인삼조감Rg1 (50 μmol·L-1)관류대조조화연폭로조대서해마뇌편20 min균능유도출LTP,연폭로조대서적LTP폭도교대조조저.재연폭로조대서적해마뇌편상,고빈자격(HFS,1 s, 100 Hz)유도적LTP교대조조현저강저, 50 μmol·L-1인삼조감Rg1관류20 min,HFS 유도적LTP폭도제고47.1%.결론 인삼조감Rg1능구제고기출적돌촉전체,병능부분수복연손상적HFS-LTP.
AIM To investigate whether ginsenoside Rg1 can reverse chronic lead-induced impairment of long-term potentiation (LTP) in the CA1 region of rat hippocampus. METHODS Neonatal Wistar rats were exposed to lead from parturition to weaning via milk of dams whose drinking water (20 mL per day) contained 0.2% lead acetate. Field excitatory postsynaptic potentials (fEPSP) were recorded and LTP was induced in the CA1 region in rat hippocampal slices on postnatal 20-25 d. RESULTS In hippocampal slices from both control and lead-exposed rats, perfusion with ginsenoside Rg1 50 μmol·L-1 for 20 min induced enhancement of fEPSP (LTP), while the amplitude of LTP in lead-exposed rats was lower than that of controls. In hippocampal slices from chronic lead-exposed rats, LTP induced by high-frequency stimulation (HFS, 1s, 100 Hz) was significantly reduced, while perfusing with ginsenoside Rg1 (50 μmol·L-1) for 20 min increased the amplitudes of LTP induced by HFS by 47.1%. CONCLUSION Rg1 can increase basic synaptic transmission and partially reverse chronic lead-induced impairment of HFS-LTP.