军事医学科学院院刊
軍事醫學科學院院刊
군사의학과학원원간
BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES
2009年
6期
550-553
,共4页
地黄属%梓醇%大孔吸附树脂%分离纯化
地黃屬%梓醇%大孔吸附樹脂%分離純化
지황속%재순%대공흡부수지%분리순화
Rehmannia%catalpol%macroporous adsorption resin%separation and purification
目的:选择合适的大孔吸附树脂对鲜地黄叶中梓醇进行富集纯化.方法:以高效液相为检测手段,梓醇含量为检测指标.采用吸附和解吸试验考察了12种大孔吸附树脂对梓醇的吸附解吸性能,筛选出H103树脂进行了分离纯化研究.结果:H103树脂对梓醇具有很好的吸附解吸性能,其纯化条件为上样液浓度1 mg/ml,上样体积2 BV,上样吸附速度1～2 BV/h,洗脱速度1～3 BV/h,10%乙醇洗脱8 BV.结论:采用H103树脂分离纯化鲜地黄叶中梓醇,可简化步骤,降低成本,方法简单可行.
目的:選擇閤適的大孔吸附樹脂對鮮地黃葉中梓醇進行富集純化.方法:以高效液相為檢測手段,梓醇含量為檢測指標.採用吸附和解吸試驗攷察瞭12種大孔吸附樹脂對梓醇的吸附解吸性能,篩選齣H103樹脂進行瞭分離純化研究.結果:H103樹脂對梓醇具有很好的吸附解吸性能,其純化條件為上樣液濃度1 mg/ml,上樣體積2 BV,上樣吸附速度1～2 BV/h,洗脫速度1～3 BV/h,10%乙醇洗脫8 BV.結論:採用H103樹脂分離純化鮮地黃葉中梓醇,可簡化步驟,降低成本,方法簡單可行.
목적:선택합괄적대공흡부수지대선지황협중재순진행부집순화.방법:이고효액상위검측수단,재순함량위검측지표.채용흡부화해흡시험고찰료12충대공흡부수지대재순적흡부해흡성능,사선출H103수지진행료분리순화연구.결과:H103수지대재순구유흔호적흡부해흡성능,기순화조건위상양액농도1 mg/ml,상양체적2 BV,상양흡부속도1～2 BV/h,세탈속도1～3 BV/h,10%을순세탈8 BV.결론:채용H103수지분리순화선지황협중재순,가간화보취,강저성본,방법간단가행.
Objective:To obtain the optimal conditions for separating catalpol from leaves of Rehmannia by selecting appropriate macroporous adsorption resins.Methods:The detection indication was the content of catalpol, which was determined by HPLC method. Twelve different kinds of macroporous adsorption resins were studied on the static capacity of adsorption and desorption, and H103 resin was selected for the research of separation and purification.Results:The H103 resin had a good capacity for adsorption and desorption.The best process of purifying catalpol by H103 resin was 1mg/ml concentration, the adsorption rate of 1-2 BV/h,the flow rate of 1-3 BV/h, and 8 BV with 10% alcohol.Conclusion:The method is simple and available, which can simplify the production process and lower costs.
