中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2011年
8期
754-759
,共6页
冬凌草甲素%PC-3细胞%增殖%抑制%凋亡
鼕凌草甲素%PC-3細胞%增殖%抑製%凋亡
동릉초갑소%PC-3세포%증식%억제%조망
oridonin%PC-3 cell%proliferation%inhibition%apoptosis
目的:探讨冬凌草甲素抑制人雄激素非依赖性前列腺癌细胞株PC-3细胞的增殖、诱导其凋亡的作用.方法:用不同浓度的冬凌草甲素干预PC-3细胞,通过MTT实验和细胞的药物浓度-时间生长曲线分析观察其对PC-3细胞活力的影响；用流式细胞仪分析PC-3早期凋亡细胞的百分率；Western印迹检测Bax Bcl-2和caspase-3蛋白表达的变化.结果:冬凌草甲素呈时间和浓度依赖性地抑制PC-3细胞增殖,药物抑制PC-3细胞活力的IC50约为10.29 μmol/L；凋亡细胞形态学鉴定、流式细胞仪检测结果均表明冬凌草甲素能以浓度依赖性方式诱导PC-3细胞凋亡(P＜0.05)；冬凌草甲素以浓度依赖性方式抑制PC-3细胞的Bcl-2蛋白表达,而上调Bax蛋白表达并活化caspaase-3.结论:冬凌草甲素可能通过线粒体途径诱导PC-3细胞凋亡.
目的:探討鼕凌草甲素抑製人雄激素非依賴性前列腺癌細胞株PC-3細胞的增殖、誘導其凋亡的作用.方法:用不同濃度的鼕凌草甲素榦預PC-3細胞,通過MTT實驗和細胞的藥物濃度-時間生長麯線分析觀察其對PC-3細胞活力的影響；用流式細胞儀分析PC-3早期凋亡細胞的百分率；Western印跡檢測Bax Bcl-2和caspase-3蛋白錶達的變化.結果:鼕凌草甲素呈時間和濃度依賴性地抑製PC-3細胞增殖,藥物抑製PC-3細胞活力的IC50約為10.29 μmol/L；凋亡細胞形態學鑒定、流式細胞儀檢測結果均錶明鼕凌草甲素能以濃度依賴性方式誘導PC-3細胞凋亡(P＜0.05)；鼕凌草甲素以濃度依賴性方式抑製PC-3細胞的Bcl-2蛋白錶達,而上調Bax蛋白錶達併活化caspaase-3.結論:鼕凌草甲素可能通過線粒體途徑誘導PC-3細胞凋亡.
목적:탐토동릉초갑소억제인웅격소비의뢰성전렬선암세포주PC-3세포적증식、유도기조망적작용.방법:용불동농도적동릉초갑소간예PC-3세포,통과MTT실험화세포적약물농도-시간생장곡선분석관찰기대PC-3세포활력적영향；용류식세포의분석PC-3조기조망세포적백분솔；Western인적검측Bax Bcl-2화caspase-3단백표체적변화.결과:동릉초갑소정시간화농도의뢰성지억제PC-3세포증식,약물억제PC-3세포활력적IC50약위10.29 μmol/L；조망세포형태학감정、류식세포의검측결과균표명동릉초갑소능이농도의뢰성방식유도PC-3세포조망(P＜0.05)；동릉초갑소이농도의뢰성방식억제PC-3세포적Bcl-2단백표체,이상조Bax단백표체병활화caspaase-3.결론:동릉초갑소가능통과선립체도경유도PC-3세포조망.
Objective To observe the proliferation inhibition and apoptosis promotion effect of oridonin on PC-3 cells.Methods PC-3 cells were treated with different concentrations of oridonin.MTT assay and drug concentration-time survival curve were used to test the effect of oridonin on the PC-3 cells.The percentage of earlier apoptosis cells was analyzed by flow cytometry.The protein expression of caspase-3,Bcl-2,and Bax in the PC-3 cells was detected by Western blot.Results Oridonin effectively inhibited the proliferation of PC-3 cells in both concentration- and time-dependent manner,and the IC50 of PC-3 cells was 10.29 μmol/L.Hochest33258 staining and flow eytometry deteced that oridonin induced the apoptosis of PC-3 cells in a concentration-dependent manner (P ＜ 0.05 ).Oridonin down-regulated Bcl-2,up-regulated Bax protein,and activated caspase-3 in a concentration-dependent manner in the PC-3 cells.Conclusion The apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.
