中华神经外科疾病研究杂志
中華神經外科疾病研究雜誌
중화신경외과질병연구잡지
CHINESE JOURNAL OF NEUROSURGICAL DISEASE RESEARCH
2012年
2期
122-127
,共6页
卒中%脑缺血%抽搐(癫痫)%海马%模型%大鼠
卒中%腦缺血%抽搐(癲癇)%海馬%模型%大鼠
졸중%뇌결혈%추휵(전간)%해마%모형%대서
Stroke%Cerebral ischemia%Seizures (epilepsy)%Hippocampus%Model%Rat
目的 为了更有效模仿临床脑缺血后发生抽搐的情况,深入研究缺血性脑损伤,在成年大鼠建立一种缺血诱发的抽搐模型.方法 分离双侧颈总动脉和双侧椎动脉,将动物体温保持在39.5～40.5℃之间,用小动脉夹依次夹闭上述四条动脉15 min,在恢复血流后动物继续维持在此温度15 min,然后移至室温.对照组大鼠仅接受手术,不做缺血处理；利用行为学观察法、组织病理学方法和脑电监护法评价两组实验动物清醒后的差异.结果 接受缺血处理的大鼠100％发生了抽搐,海马细胞广泛受损,脑电图显示大鼠发生抽搐；对照组大鼠未见异常.结论 该方法是一种简便易行的可靠的使大鼠发生脑缺血后抽搐的模型操作方法.
目的 為瞭更有效模倣臨床腦缺血後髮生抽搐的情況,深入研究缺血性腦損傷,在成年大鼠建立一種缺血誘髮的抽搐模型.方法 分離雙側頸總動脈和雙側椎動脈,將動物體溫保持在39.5～40.5℃之間,用小動脈夾依次夾閉上述四條動脈15 min,在恢複血流後動物繼續維持在此溫度15 min,然後移至室溫.對照組大鼠僅接受手術,不做缺血處理；利用行為學觀察法、組織病理學方法和腦電鑑護法評價兩組實驗動物清醒後的差異.結果 接受缺血處理的大鼠100％髮生瞭抽搐,海馬細胞廣汎受損,腦電圖顯示大鼠髮生抽搐；對照組大鼠未見異常.結論 該方法是一種簡便易行的可靠的使大鼠髮生腦缺血後抽搐的模型操作方法.
목적 위료경유효모방림상뇌결혈후발생추휵적정황,심입연구결혈성뇌손상,재성년대서건립일충결혈유발적추휵모형.방법 분리쌍측경총동맥화쌍측추동맥,장동물체온보지재39.5～40.5℃지간,용소동맥협의차협폐상술사조동맥15 min,재회복혈류후동물계속유지재차온도15 min,연후이지실온.대조조대서부접수수술,불주결혈처리；이용행위학관찰법、조직병이학방법화뇌전감호법평개량조실험동물청성후적차이.결과 접수결혈처리적대서100％발생료추휵,해마세포엄범수손,뇌전도현시대서발생추휵；대조조대서미견이상.결론 해방법시일충간편역행적가고적사대서발생뇌결혈후추휵적모형조작방법.
Objective To establish a cerebral ischemia-induced seizures model in mature SD rats and probe into the ischemic brain injury.Methods Male Sprague-Dawley rats were divided into two groups,sham group and ischemia-induced epilepsy group.The rats of the ischemia-induced epilepsy group were exposed to 4-vessel occlusion when the body temperature was heated at 39.5 ℃ to 40.5 ℃.The rats of sham group underwent the same procedures with the exception of the occlusion.The rat condition of two groups was evaluated and compared statistically.Results All the rats of epilepsy group showed a significantly stable,symmetrical pycnosis charges in bilateral hippocampus and cortexes; seizure activity was scored by seizure rating scale and electroencephalograph.Conclusion This model is a stable and easy getting method for acute ischemia-induced seizures or even a model for the severe brain ischemia injury.
