CAJ | 학술논문

目的研究富勒烯衍生物C3(以下简称C3)对AHH-1细胞电离辐射的防护效应,探讨该类化合物作为新型辐射防护剂的发展前景.方法通过化学合成制备C3,用台盼兰拒染试验检测C3对AHH-1细胞的毒性作用.在此基础上于AHH-1细胞培养体系中加入不同浓度C3,以不同剂量60Coγ射线照射细胞,通过台盼兰拒染试验检测C3化合物对细胞的毒性作用,用Annexin-V/PI染色、Facscalibur流式细胞术等,分析C3化合物对γ射线照射细胞增殖活力和细胞凋亡的变化.结果在细胞培养体系中,终浓度范围0～400 mg/L的C3对培养的对数生长期AHH-1细胞活力几乎无影响,AHH-1细胞台盼兰拒染率无明显变化(P＞0.05).C3对1～8 Gy γ射线照射的AHH-1细胞具有良好的辐射防护效应,终浓度在10 mg/L时即显示其对4 Gy60Co γ照射的细胞有保护效应,且随浓度增加而增加;终浓度达200～400 mg/L时,照射细胞存活率已接近未照射的正常细胞(P＞0.05),辐射诱导的细胞调亡率和细胞死亡率用药组均显著低于未用药的对照组(P＜0.01).研究结果还显示,C3提高照射细胞存活率的作用还与给药时间有关,照前24 h至照前即刻给药最为有效.结论 C3对不同剂量60Co γ射线照射的AHH-1细胞具有较好的电离辐射防护作用,该防护效应与用药浓度有关,C3浓度越高,防护作用越好;且在本实验产生辐射防护作用的浓度范围内,C3对培养的对数生长期AHH-1细胞存活率几乎无影响,提示该类化合物有作为新型辐射防护剂的研究前景.
목적 연구부륵희연생물C3(이하간칭C3)대AHH-1세포전리복사적방호효응,탐토해류화합물작위신형복사방호제적발전전경.방법 통과화학합성제비C3,용태반란거염시험검측C3대AHH-1세포적독성작용.재차기출상우AHH-1세포배양체계중가입불동농도C3,이불동제량60Coγ사선조사세포,통과태반란거염시험검측C3화합물대세포적독성작용,용Annexin-V/PI염색、Facscalibur류식세포술등,분석C3화합물대γ사선조사세포증식활력화세포조망적변화.결과 재세포배양체계중,종농도범위0～400 mg/L적C3대배양적대수생장기AHH-1세포활력궤호무영향,AHH-1세포태반란거염솔무명현변화(P＞0.05).C3대1～8 Gy γ사선조사적AHH-1세포구유량호적복사방호효응,종농도재10 mg/L시즉현시기대4 Gy60Co γ조사적세포유보호효응,차수농도증가이증가;종농도체200～400 mg/L시,조사세포존활솔이접근미조사적정상세포(P＞0.05),복사유도적세포조망솔화세포사망솔용약조균현저저우미용약적대조조(P＜0.01).연구결과환현시,C3제고조사세포존활솔적작용환여급약시간유관,조전24 h지조전즉각급약최위유효.결론 C3대불동제량60Co γ사선조사적AHH-1세포구유교호적전리복사방호작용,해방호효응여용약농도유관,C3농도월고,방호작용월호;차재본실험산생복사방호작용적농도범위내,C3대배양적대수생장기AHH-1세포존활솔궤호무영향,제시해류화합물유작위신형복사방호제적연구전경.
Objective To investigate the radio-protective effects of carboxy fullerene C3(C3)on AHH-1 cell.Methods C3 was prepared by chemical synthesis and trypan blue rejection test was performed to detect the cytotoxicity of AHH-1 cell.Then different concentration of C3 Wag used to treat AHH-1 cells radiated with 60Co γ ray.Annexin-V/PI staining and flow cytometry assay were applied to assess the proliferation and apoptosis of cell after irradiation.Results In cell culture system,C3 showed little toxicity to AHH-1 cells with little change of trypan blue rejection rate within the drug concentration range 0-400 mg/L(P＞0.05).We found in this study C3 had good radio-protection effects to AHH-1 cell irradiated with 1-8 Gy γ-ray.When the concentration was 10 mg/L,C3 showed protective effects to AHH-1 cell irradiated with 4 Gy γ-ray,which was enhanced with increasing C3 concentration.When the final concentration reached 200-400 mg/L,the cell survival rate after irradiation was similar to that of non-irradiated control cell(P＞0.05).And the irradiation induced apoptosis and death rate were significantly lower than those of single irradiated group cells(P＜0.01).Moreover,the radio-protective effects of C3 were time-dependant,and the best protection effects were observed when the C3 was administered before irradiation(0-24 h).Conclusions C3 has good radioprotective effects to AHH-1 cell,which is dose-dependent,and the higher concentration of C3 is,the better protective effect showes.in the effective drug concentration range of this study.C3 does little harm on the survival rate of AHH-1 cell which suggests that C3 as a novel promising radio-protectant deserve to be fuaher investigated.