中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2008年
2期
125-128
,共4页
张鑫%崔瑾%邱明才%李德强%张鹏%张金时
張鑫%崔瑾%邱明纔%李德彊%張鵬%張金時
장흠%최근%구명재%리덕강%장붕%장금시
糖尿病视网膜病%免疫球蛋白类%环孢菌素
糖尿病視網膜病%免疫毬蛋白類%環孢菌素
당뇨병시망막병%면역구단백류%배포균소
Diabetic retinopathy%Immunoglobulins%Cyelosporine
目的 探讨糖尿病视网膜病变(DR)发病过程中的免疫机制,观察环孢素A(CsA)对糖尿病大鼠视网膜病变的防治作用.方法 以链脲菌素诱导的糖尿病大鼠为模型,分别于造模前或成模后给予低、中、高剂量CsA皮下注射,并设立正常、单纯糖尿病和胰岛素治疗组为对照,通过HE染色观察糖尿病大鼠视网膜病变,通过免疫组化和免疫荧光检测视网膜免疫球蛋白的沉积.结果 8周时,糖尿病大鼠视网膜已经出现DR的病理学表现,且有大量IgG、IgA、IgM沉积.胰岛素治疗组免疫球蛋白沉积情况与单纯糖尿病组差异无统计学意义.CsA干预各组的视网膜免疫球蛋白沉积情况与单纯糖尿病组相比差异有统计学意义(P<0.05),尤以高剂量组为著(P<0.01).结论 免疫机制参与DR的发生,免疫抑制治疗能有效地减少视网膜免疫球蛋白沉积,延缓DR的发生.
目的 探討糖尿病視網膜病變(DR)髮病過程中的免疫機製,觀察環孢素A(CsA)對糖尿病大鼠視網膜病變的防治作用.方法 以鏈脲菌素誘導的糖尿病大鼠為模型,分彆于造模前或成模後給予低、中、高劑量CsA皮下註射,併設立正常、單純糖尿病和胰島素治療組為對照,通過HE染色觀察糖尿病大鼠視網膜病變,通過免疫組化和免疫熒光檢測視網膜免疫毬蛋白的沉積.結果 8週時,糖尿病大鼠視網膜已經齣現DR的病理學錶現,且有大量IgG、IgA、IgM沉積.胰島素治療組免疫毬蛋白沉積情況與單純糖尿病組差異無統計學意義.CsA榦預各組的視網膜免疫毬蛋白沉積情況與單純糖尿病組相比差異有統計學意義(P<0.05),尤以高劑量組為著(P<0.01).結論 免疫機製參與DR的髮生,免疫抑製治療能有效地減少視網膜免疫毬蛋白沉積,延緩DR的髮生.
목적 탐토당뇨병시망막병변(DR)발병과정중적면역궤제,관찰배포소A(CsA)대당뇨병대서시망막병변적방치작용.방법 이련뇨균소유도적당뇨병대서위모형,분별우조모전혹성모후급여저、중、고제량CsA피하주사,병설립정상、단순당뇨병화이도소치료조위대조,통과HE염색관찰당뇨병대서시망막병변,통과면역조화화면역형광검측시망막면역구단백적침적.결과 8주시,당뇨병대서시망막이경출현DR적병이학표현,차유대량IgG、IgA、IgM침적.이도소치료조면역구단백침적정황여단순당뇨병조차이무통계학의의.CsA간예각조적시망막면역구단백침적정황여단순당뇨병조상비차이유통계학의의(P<0.05),우이고제량조위저(P<0.01).결론 면역궤제삼여DR적발생,면역억제치료능유효지감소시망막면역구단백침적,연완DR적발생.
Objective To study the autoimmune injuries on diabetic retinopathy(DR)and the protective effects of immunosuppre8sive therapy with eyelosporine A(CsA) on the DR of streptozotocin(STZ)-induced diabetic rats.Methods STZ-induced diabetic rats were randomly divided into 3 groups: group 1:diabetic group without any treatment;group 2:insulin-treated group;group 3:CsA-treated group which was further divided into 2 subgroups:subgroup A:CsA was given 1 week before hyperglycemia appeared and subgroup B:CsA was given one week after hyperglycemia appeared.Subgroup A and subgroup B were further subdivided into 3 groups respectively,based on the dose of CsA(1,4 and 8 mg·kg-1·d-1).As a control group,normal rats were also simultaneously monitored.The pathologic changes in the retina were investigated with HE stain and the deposition of immunoglobulins was detected with immunohistochemistry and immunofluorescent microscopy.Results After 8 week,the deposition of IgG, IgA and IgM was quite significant in the retina of diabetic rats.The data also suggested that insulin treatment had no effects on the DR.In contrast,with CsA intervention,the deposition of immunoglobulins on the retina of diabetic rats vanished.Conclusions Autoimmune injuries were shown,in the present study,to play a critical role in the pathogenesis of diabetic retinopathy.Immunosuppressive treatment with CsA showed protective effects by inhibiting the deposition of immunoglobulins in the retina of diabetic rats.