CAJ | 학술논문

目的探讨染色体异常在肺癌发生和发展中的可能作用.方法采用比较基因组杂交(CGH)技术分析了17例原发性肺癌新鲜组织的染色体异常.结果 17例肺癌组织均检出染色体畸变(扩增和/或缺失),而且每个病例涉及多条染色体畸变.非小细胞肺癌(NSCLC)的染色体扩增和缺失平均发生率(7处/例和4.8处/例)与小细胞肺癌(SCLC)(8.4处/例和9.6处/例)差异无统计学意义,但NSCLC与SCLC的染色体扩增和缺失的发生部位及其频率有所不同,NSCLC染色体3q24- 28和11q13的扩增的频率(58.3％和58.3％)显著高于SCLC(0％和0％)(P＜0.05).结论 NSCLC和SCLC在发生和发展过程中都涉及多部位染色体(多基因)异常,NSCLC与SCLC染色体扩增和缺失的发生部位及其频率有所不同可能是导致两者不同生物学特性的基础.
목적 탐토염색체이상재폐암발생화발전중적가능작용.방법 채용비교기인조잡교(CGH)기술분석료17례원발성폐암신선조직적염색체이상.결과 17례폐암조직균검출염색체기변(확증화/혹결실),이차매개병례섭급다조염색체기변.비소세포폐암(NSCLC)적염색체확증화결실평균발생솔(7처/례화4.8처/례)여소세포폐암(SCLC)(8.4처/례화9.6처/례)차이무통계학의의,단NSCLC여SCLC적염색체확증화결실적발생부위급기빈솔유소불동,NSCLC염색체3q24- 28화11q13적확증적빈솔(58.3％화58.3％)현저고우SCLC(0％화0％)(P＜0.05).결론 NSCLC화SCLC재발생화발전과정중도섭급다부위염색체(다기인)이상,NSCLC여SCLC염색체확증화결실적발생부위급기빈솔유소불동가능시도치량자불동생물학특성적기출.
Objective To understand the molecular aberration at whole genomic level,CGH (comparative genomic hybridization) was used to investigate genetic abnormality in lung cancer.Methods Comparative genomic hybridization was performed in 17 cases to detect the global genomic aberration in cancer tissue cells.Results All of 17 cases detected by CGH showed chromosomal aberrations.The average numbers of chromosomal gains and losses in each case were 7.0 and 4.8 in NSCLC and 8.4 and 9.6 in SCLC,respectively.The frequency of gains and losses on chromosome had no significant differences between NSCLC and SCLC.The frequencies of gains on chromosomal arms 3q24 -28 and 11q13(58.3％ and 58.3％ ) in NSCLC were significantly higher than that in SCLC(0％ and 0％ ) ( P ＜0.05 and ＜0.05,respectively).Conclusions The cytogenetic aberration generally existed in lung cancer cells.Several regions ( more than one) of chromosomal aberration were involved in the carcinogenesis of NSCLC and SCLC.The regions and frequencies of chromosomal aberration in NSCLC were somewhat different from that in SCLC,which might result in the different biological behavior of the two types of lung cancer.The chromosomal aberration might be served as a marker to differentiate the two types of lung cancer.