CAJ | 학술논문

目的探讨乙型肝炎病毒基因型和DNA载量与外膜大蛋白关系.方法采用荧光定量PCR方法 ,ELISA法和时间分辨免疫荧光分析法分别检测140例慢性乙型肝炎患者血清中HBVDNA、LHBs(Hepatitis B Virus Large Envelope Protein,LHBs)和HBV血清标志物,对HBV DNA阳性标本进行基因测序鉴定基因型,并进行相关性分析.结果 HBV LHBs与HBV DNA阳性率在HBcAg阴性和阳性组中差异均无统计学意义(P＞0.05);HBV LHBs吸光度A值与HBV DNA载量存在良好的相关性,相关系数为0.9267;乙型肝炎病毒B、C基因型间HBV-LHBs吸光度A值差异无统计学意义(P＞0.05).结论血清LHBs水平与HBV DNA载量存在良好的相关性,能反映HBV感染者体内HBV复制程度,可作为判断HBV复制新的血清学指标;HBV LHBs的含量与HBV基因型无关.
목적 탐토을형간염병독기인형화DNA재량여외막대단백관계.방법 채용형광정량PCR방법 ,ELISA법화시간분변면역형광분석법분별검측140례만성을형간염환자혈청중HBVDNA、LHBs(Hepatitis B Virus Large Envelope Protein,LHBs)화HBV혈청표지물,대HBV DNA양성표본진행기인측서감정기인형,병진행상관성분석.결과 HBV LHBs여HBV DNA양성솔재HBcAg음성화양성조중차이균무통계학의의(P＞0.05);HBV LHBs흡광도A치여HBV DNA재량존재량호적상관성,상관계수위0.9267;을형간염병독B、C기인형간HBV-LHBs흡광도A치차이무통계학의의(P＞0.05).결론 혈청LHBs수평여HBV DNA재량존재량호적상관성,능반영HBV감염자체내HBV복제정도,가작위판단HBV복제신적혈청학지표;HBV LHBs적함량여HBV기인형무관.
Objective To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein. Methods Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofiuorescence assay in 140 serum samples collected from chronic hepatitis B patients. The genotypes of HBV were identified by DNA sequencing; and analyze their relationship. Results There was no significant difference between positive rate of LHBs and that of HBV DNA in HBeAg negative and positive group(P＞0.05); The HBV LHBs absorbency was markedly correlated with the HBV DNA load(R2=0.9267). The difference of HBV LHBs absorbency between HBV genotype B and C was not significant. Conclusions The close correlation between HBV LHBs absorbence and HBV DNA load illustrated that he level of serum LHBs can be used to estimate the state of HBV replication; and there is no relationship between HBV LHBs absorbency and genotypes. So HBV LHBs may be used as a new serological marker to detect HBV replication.