CAJ | 학술논문

目的采用血清蛋白质组学技术筛选并鉴定肝癌转移相关的蛋白分子.方法建立不同转移潜能人肝癌HCCLM9和MHCC97L动物模型各8只,采用双向凝胶电泳和MALDI串联飞行时间质谱仪(MALDI-TOF/TOF)技术比较和鉴定差异表达的血清蛋白分子,Western blot和动物模型标本验证后,进一步对208例临床标本(男性170例,女性38例)验证.结果 HCCLM9和MHCC97L组比较,血清蛋白表达显著差异达11个,其中补体因子H(CFH)在HCCLM9模型血清中水平(213.83±55.17)是MHCC97L组(122.48±48.91)的1.75倍.裸鼠肝癌组织中免疫组织化学提示CFH表达明显升高.208例临床病理组织显示,CFH表达越强,临床病期越晚.结论 CFH与肝癌侵袭转移相关.
목적 채용혈청단백질조학기술사선병감정간암전이상관적단백분자.방법 건립불동전이잠능인간암HCCLM9화MHCC97L동물모형각8지,채용쌍향응효전영화MALDI천련비행시간질보의(MALDI-TOF/TOF)기술비교화감정차이표체적혈청단백분자,Western blot화동물모형표본험증후,진일보대208례림상표본(남성170례,녀성38례)험증.결과 HCCLM9화MHCC97L조비교,혈청단백표체현저차이체11개,기중보체인자H(CFH)재HCCLM9모형혈청중수평(213.83±55.17)시MHCC97L조(122.48±48.91)적1.75배.라서간암조직중면역조직화학제시CFH표체명현승고.208례림상병리조직현시,CFH표체월강,림상병기월만.결론 CFH여간암침습전이상관.
Objective Serum proteomics approaches were applied to search for and validate hepatocellular carcinoma (HCC) invasion- and metastasis-related biomarkers. Methods Sixteen male athymic BALB/C nu/nu mice were randomly divided into two groups. HCCLM9-and MHCC97L-nude mice models of human HCC, with a similar genetic background and remarkably different pulmonary metastasis potential,were established. 2D gel electrophoresis (2-DE) and MALDI-TOF/TOF technologies were used to construct a comparative proteome profile of nude mice sera from HCCLM9- and MHCC97L-nude mice. Candidate protein was confirmed by Western blotting on serum, immunohistochemistry (IHC) on animal tumor tissues, and tissue micro-array on clinical specimens including 170 male cases and 38 female cases. Results Serum proteins from HCCLM9- and MHCC97L-nude mice were compared by 2-DE. A total of 11 proteins were identified by MALDI-TOF/TOF. Complement factor H (CFH) was overexpressed in HCCLM9- (213.83 ± 55.17) versus MHCC97L-nude mice serum (122.48 ±48.91 ) by Western blotting,and validated by IHC from both nude mice tumor tissues and clinical specimens. Elevated CFH expression was observed in liver cancer tissues of HCCLM9 nude mice. IHC study in 208 human HCC specimens demonstrated that patients with higher CFH expession showed greater invasiveness and more advanced stage.Conclusion CFH may be a candidate biomarker to predict HCC invasive behavior.