CAJ | 학술논문

为了提高胰岛素原的表达量,用pQE-40质粒构建了(His)6-Arg-Arg-人胰岛素原[(His)6-Arg-Arg-human Proinsulin,RRhPI]的大肠杆菌表达系统.通过培养条件的优化,在摇瓶培养的条件下,目标产物RRhPI以包涵体形式获得了高效表达,每升培养基收获湿菌体约27g,包涵体6g(干重1.8g),RRhPI约540mg.该水平已超过现有文献报道的摇瓶培养的最高水平.
위료제고이도소원적표체량,용pQE-40질립구건료(His)6-Arg-Arg-인이도소원[(His)6-Arg-Arg-human Proinsulin,RRhPI]적대장간균표체계통.통과배양조건적우화,재요병배양적조건하,목표산물RRhPI이포함체형식획득료고효표체,매승배양기수획습균체약27g,포함체6g(간중1.8g),RRhPI약540mg.해수평이초과현유문헌보도적요병배양적최고수평.
To enhance the expression level of human proinsulin, Escherichia coli ( E. coli ) M15 harbouring a plasmid(His)6-Arg -Arg -human proinsulin (RRhPI)/pQE-40 for human proinsulin production was cultured using flask. As a result,high expression level of the gene product, which accumulated as inclusion bodies, was obtained. 27g wet E. coli cells wereharvested from 1L culture medium, and 6g inclusion body also. The harvest of our designed gene product RRhPI was about540mg/L culture medium.