CAJ | 학술논문

目的探讨氧化苦参碱(OMT)对二硝基氟苯(DNFB)所致小鼠变应性接触性皮炎(ACD)的抑制作用及小鼠淋巴细胞增殖的影响.方法建立DNFB所致小鼠ACD模型,以不同剂量的OMT、PBS、氢化可的松(HCT)进行腹腔注射,检测小鼠耳廓肿胀度变化;利用羧基荧光素乙酰乙酸(CFDA-SE)染色,流式细胞术检测OMT对小鼠淋巴细胞增殖的影响.结果 OMT对DNFB所致小鼠ACD呈剂量依赖性抑制作用,且与同等剂量HCT作用效果相似,但副作用明显减小;体外实验证明,在500、125和31 μg/mL组OMT对小鼠淋巴细胞增殖呈剂量依赖性抑制.结论 OMT对DNFB所致小鼠ACD有显著的抑制作用,而且抑制小鼠淋巴细胞增殖;OMT是一种免疫抑制剂.
목적 탐토양화고삼감(OMT)대이초기불분(DNFB)소치소서변응성접촉성피염(ACD)적억제작용급소서림파세포증식적영향.방법 건립DNFB소치소서ACD모형,이불동제량적OMT、PBS、경화가적송(HCT)진행복강주사,검측소서이곽종창도변화;이용최기형광소을선을산(CFDA-SE)염색,류식세포술검측OMT대소서림파세포증식적영향.결과 OMT대DNFB소치소서ACD정제량의뢰성억제작용,차여동등제량HCT작용효과상사,단부작용명현감소;체외실험증명,재500、125화31 μg/mL조OMT대소서림파세포증식정제량의뢰성억제.결론 OMT대DNFB소치소서ACD유현저적억제작용,이차억제소서림파세포증식;OMT시일충면역억제제.
[Objective] To explore the restraint-effect of oxymatrine (OMT)on allergic contact dermatitis (ACD)and lymphocyte proliferation. [Methods] 1.To build up an ACD mouse model stimulated by DNFB and then to perform intraperitoneal injection with different dosages of OMT, PBS and hydrocortisone(HCT), To observe the curative effect by examining the swelling degree of mice auricles. 2. Dyed by Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) and then flow cytometer was used to examine the fluorescence intensity changes of lymphocyte co-influenced by polyclonal stimulator Concanavalin A (Con A) and OMT. Whereafter, analyze the efficacy of OMT on mouse lymphocyte proliferation by related software. [Results] 1. Comparing with PBS group, OMT possesses much stronger restraint-effect on ACD that caused by DNFB and depends on its injection dosage. Its restraint-effect is equivalent to the HCT of the same dosage, but brings fewer side effects. 2. In vitro expenment, it proves that OMT has the effects to restrain the proliferation of mice's lymphocyte depending on its concentration in substrate as it behaves differently in group 500, 125 and 31 μg/ml. [Conclusion] OMT possesses the obvious effect of restraining the ACD stimulated by DNFB; OMT is a kind of immunosuppressor.