CAJ | 학술논문

目的检验猫眼草水煎液的致突变性;改进经典的Ames试验体系使之适应于中药体外致突变性检验.方法通过经典的Ames试验检测猫眼草的体外致突变性;通过哺乳动物骨髓细胞染色体畸变试验检测猫眼草的致畸作用;改进的Ames试验通过增设含补充组氨酸(含量对应于猫眼草水煎液中组氨酸浓度)的阴性对照,排除样品中组氨酸成分对试验结果的影响.结果猫眼草水煎液在经典的Ames试验中为强阳性;对哺乳动物骨髓细胞染色体致畸作用为阴性;在改进的Ames试验中猫眼草水煎液的致突变性为阴性.结论经典的Ames试验不适合猫眼草水煎液致突变性检测,改进后的Ames实验体系适合.猫眼草水煎液在体外和体内均没有致突变性.
목적 검험묘안초수전액적치돌변성;개진경전적Ames시험체계사지괄응우중약체외치돌변성검험.방법 통과경전적Ames시험검측묘안초적체외치돌변성;통과포유동물골수세포염색체기변시험검측묘안초적치기작용;개진적Ames시험통과증설함보충조안산(함량대응우묘안초수전액중조안산농도)적음성대조,배제양품중조안산성분대시험결과 적영향.결과 묘안초수전액재경전적Ames시험중위강양성;대포유동물골수세포염색체치기작용위음성;재개진적Ames시험중묘안초수전액적치돌변성위음성.결론 경전적Ames시험불괄합묘안초수전액치돌변성검측,개진후적Ames실험체계괄합.묘안초수전액재체외화체내균몰유치돌변성.
Aim To assay the mutagenicity of Enphorbia lunulata(EL) decoction and to modify the Ames test for evaluation the mutagenicity of herbal medicine samples.Method The mutagenicity of EL decoction was assayed by standard Ames test; the teratogenicity was done by mammalian bone marrow chromosomal aberration test. In modified Ames test system,the influence of histidine EL decoction was excluded by additional negative control, in which the test media was supplied with histidine (histidine amount equaled to the histidine in different concentration of EL decoction).Result The mutagenicity of EL decoction was positive in the standard Ames test. The teratogenicity of EL decoction was negative in mammalian bone marrow chromosomal aberration test. By the modified Ames tests,the mutagenicity of EL decoction was negative.Conclusion The standard Ames test is not suitable for evaluating the mutagenicity of EL decoction, but the modified Ames test is. The mutagenicity in vitro and the teratogenicity in vivo of EL decoction are all negative.