CAJ | 학술논문

运用电子定位的方法,利用大豆基因组物理图和遗传图的整合图谱,完成了17个大豆天冬氨酸代谢途径中关键酶基因的定位以及结构分析.结果表明:这些酶基因分别定位在A1、B2、D1a、D1b、D2、F、G、H、J、L和N等11个连锁群上,并获得了相应连锁群区间两侧的标记;在基因结构分析上,AHAS、DHDPS、HK与TS没有内含子,DHAD基因的内含子数目最多,为13个,AK基因有12个内含子.通过定位获得的对应分子标记可以为基因分子辅助育种奠定基础,而基因结构信息能更好的实现基因功能分析.
운용전자정위적방법,이용대두기인조물리도화유전도적정합도보,완성료17개대두천동안산대사도경중관건매기인적정위이급결구분석.결과표명:저사매기인분별정위재A1、B2、D1a、D1b、D2、F、G、H、J、L화N등11개련쇄군상,병획득료상응련쇄군구간량측적표기;재기인결구분석상,AHAS、DHDPS、HK여TS몰유내함자,DHAD기인적내함자수목최다,위13개,AK기인유12개내함자.통과정위획득적대응분자표기가이위기인분자보조육충전정기출,이기인결구신식능경호적실현기인공능분석.
The nutritive value of soybean mainly focused on the aspartate-derived amino acids,and the content of these amino acids were affected by the key enzymes gene from aspartic acid metabolic pathway.In this research,the integrated map between soybean public genetic maps and physical maps was used,and the key enzyme gene sequences were blasted with soybean genome database by local Blast software.Seventeen key enzyme genes in aspartic acid metabolic pathway of soybean were mapped on soybean genetic map,and gene structures were analyzed firstly.The result indicated that these enzyme genes were mapped on 11 linkages group,including A2,B2,C2,D1b,D2,G,I,L,and M,respectively,and the flanking markers of these genes on the linkage group were obtained.The number of introns was from 0 to 13.There was no intron in AHAS,DHDPS,HK,and TS,otherwise there were 13 introns in DHAD,and 12 introns in AK.The corresponding markers obtained from mapping were the base of molecular assisted selection,while the structure information can make gene function analysis easily.